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Literature summary for 3.4.17.13 extracted from

  • Das, D.; Herve, M.; Elsliger, M.A.; Kadam, R.U.; Grant, J.C.; Chiu, H.J.; Knuth, M.W.; Klock, H.E.; Miller, M.D.; Godzik, A.; Lesley, S.A.; Deacon, A.M.; Mengin-Lecreulx, D.; Wilson, I.A.
    Structure and function of a novel LD-carboxypeptidase a involved in peptidoglycan recycling (2013), J. Bacteriol., 195, 5555-5566.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.17.13

Crystallization (Commentary)

Crystallization (Comment) Organism
to 1.89 A resolution, abd modeling of interaction with substrate Novosphingobium aromaticivorans

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibitory: EDTA at 2.5 mM Novosphingobium aromaticivorans
Zn2+ 2 mM, more than 95% inhibition Novosphingobium aromaticivorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
 L-Ala-gamma-D-Glu-meso-diaminopimelyl-D-Ala pH 8.0, 37°C Novosphingobium aromaticivorans

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no divalent cation required Novosphingobium aromaticivorans

Organism

Organism UniProt Comment Textmining
Novosphingobium aromaticivorans Q2G8F3
-
-
Novosphingobium aromaticivorans DSM 12444 Q2G8F3
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
24.5
-
 substrate UDP-N-acetylmuramyl-L-Ala-gamma-D-Glu-meso-diaminopimelyl-D-Ala, pH 8.0, 37°C Novosphingobium aromaticivorans
30
-
 substrate N-acetylmuramyl-L-Ala-D-Glu-meso-diaminopimelyl-D-Ala, pH 8.0, 37°C Novosphingobium aromaticivorans
230
-
 substrate L-Ala-gamma-D-Glu-meso-diaminopimelyl-D-Ala, pH 8.0, 37°C Novosphingobium aromaticivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala-gamma-D-Glu-meso-diaminopimelyl-D-Ala + H2O
-
 Novosphingobium aromaticivorans L-Ala-gamma-D-Glu-meso-diaminopimelyl + D-Ala
-
 ?
L-Ala-gamma-D-Glu-meso-diaminopimelyl-D-Ala + H2O
-
 Novosphingobium aromaticivorans DSM 12444 L-Ala-gamma-D-Glu-meso-diaminopimelyl + D-Ala
-
 ?
additional information enzyme prefers tetrapeptide substrates and can discriminate between tetrapeptide and pentapeptide substrates, but is less selective toward the N-terminal sugar moiety. No substrate: UDP-N-acetylmuramyl-L-Ala-gamma-D-Glu-meso-diaminopimelyl-D-Ala-D-Ala, poor substrate: N-acetylmuramyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala Novosphingobium aromaticivorans ?
-
 ?
additional information enzyme prefers tetrapeptide substrates and can discriminate between tetrapeptide and pentapeptide substrates, but is less selective toward the N-terminal sugar moiety. No substrate: UDP-N-acetylmuramyl-L-Ala-gamma-D-Glu-meso-diaminopimelyl-D-Ala-D-Ala, poor substrate: N-acetylmuramyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala Novosphingobium aromaticivorans DSM 12444 ?
-
 ?
N-acetylmuramyl-L-Ala-D-Glu-meso-diaminopimelyl-D-Ala + H2O
-
 Novosphingobium aromaticivorans N-acetylmuramyl-L-Ala-D-Glu-meso-diaminopimelate + D-Ala
-
 ?
N-acetylmuramyl-L-Ala-D-Glu-meso-diaminopimelyl-D-Ala + H2O
-
 Novosphingobium aromaticivorans DSM 12444 N-acetylmuramyl-L-Ala-D-Glu-meso-diaminopimelate + D-Ala
-
 ?
UDP-N-acetylmuramyl-L-Ala-gamma-D-Glu-meso-diaminopimelyl-D-Ala + H2O
-
 Novosphingobium aromaticivorans UDP-N-acetylmuramyl-L-Ala-D-Glu-meso-diaminopimelate + D-Ala
-
 ?
UDP-N-acetylmuramyl-L-Ala-gamma-D-Glu-meso-diaminopimelyl-D-Ala + H2O
-
 Novosphingobium aromaticivorans DSM 12444 UDP-N-acetylmuramyl-L-Ala-D-Glu-meso-diaminopimelate + D-Ala
-
 ?

Synonyms

Synonyms Comment Organism
LdcA
-
 Novosphingobium aromaticivorans
peptidase U61
-
 Novosphingobium aromaticivorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
160
-
 L-Ala-gamma-D-Glu-meso-diaminopimelyl-D-Ala pH 8.0, 37°C Novosphingobium aromaticivorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4 7.6
-
 Novosphingobium aromaticivorans