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Literature summary for 3.4.17.12 extracted from

  • Zhang, X.; Tan, F.; Zhang, Y.; Skidgel, R.A.
    Carboxypeptidase M and kinin B1 receptors interact to facilitate efficient B1 signaling from B2 agonists (2008), J. Biol. Chem., 283, 7994-8004.
    View publication on PubMed
Search for more literature for 3.4.17.12

Cloned(Commentary)

Cloned (Comment) Organism
CPM cDNAs are cloned into pcDNA3 or pcDNA6 for expression in mammalian cells, to express N-terminal CFP- or YFP-tagged CPM pECFP-C1 and pEYFP-C1 vectors are used, to generate a fusion protein of CPM attached to the extracellular N-terminus of B1R pcDNA3 and 6 vectors are used Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 bradykinin purified CPM in vitro Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
 Homo sapiens 5886
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dansyl-Ala-Arg + H2O substrate for determination of CPM activity Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
carboxypeptidase M
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 activity assay Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.45
-
 bradykinin purified CPM in vitro Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 neutral pH optimum Homo sapiens