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Literature summary for 3.4.17.12 extracted from

  • Deiteren, K.; Surpateanu, G.; Gilany, K.; Willemse, J.L.; Hendriks, D.F.; Augustyns, K.; Laroche, Y.; Scharpe, S.; Lambeir, A.M.
    The role of the S1 binding site of carboxypeptidase M in substrate specificity and turn-over (2007), Biochim. Biophys. Acta, 1774, 267-277.
    View publication on PubMed
Search for more literature for 3.4.17.12

Cloned(Commentary)

Cloned (Comment) Organism
placental cDNA is incorporated in the pICZalphaA shuttle vector, signal peptide and C-terminal hydrophobic membrane anchor signal are removed Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
MERGETPA
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
 Leu-Ala-Arg CPMwt, pH 7.4, 37°C Homo sapiens
0.0277
-
 bradykinin(-Phe-Arg) CPMwt, pH 7.2, 37°C Homo sapiens
0.028
-
 His-Leu-Gly-Leu-Ala-Arg CPMwt, pH 7.4, 37°C Homo sapiens
0.033
-
 Ala-Ser-His-Leu-Gly-Leu-Ala-Arg CPMwt, pH 7.4, 37°C Homo sapiens
0.035
-
 Pro-Gly-Lys-Ala-Arg CPMwt, pH 7.2, 37°C Homo sapiens
0.041
-
 Leu-Gly-Leu-Ala-Arg CPMwt, pH 7.4, 37°C Homo sapiens
0.046
-
 benzoyl-Gly-argininic acid CPMwt, pH 7.2, 37°C Homo sapiens
0.048
-
 benzoyl-Tyr-Arg CPMwt, pH 7.4, 37°C Homo sapiens
0.049
-
 benzoyl-Gly-argininic acid hCPM, pH 7.2, 37°C Homo sapiens
0.05
-
 Gly-Leu-Ala-Arg CPMwt, pH 7.4, 37°C Homo sapiens
0.051
-
 Ser-His-Leu-Gly-Leu-Ala-Arg CPMwt, pH 7.4, 37°C Homo sapiens
0.0584
-
 benzoyl-Phe-Arg CPMwt, pH 7.4, 37°C Homo sapiens
0.068
-
 furylacryloyl-Ala-Arg hCPM, pH 7.2, 37°C Homo sapiens
0.069
-
 benzoyl-Trp-Arg CPMwt, pH 7.4, 37°C Homo sapiens
0.073
-
 Dansyl-Ala-Arg hCPM, pH 7.2, 37°C Homo sapiens
0.083
-
 Dansyl-Ala-Arg CPMwt, pH 7.2, 37°C Homo sapiens
0.09
-
 furylacryloyl-Ala-Arg CPMwt, pH 7.2, 37°C Homo sapiens
0.115
-
 benzoyl-Ala-Arg CPMwt, pH 7.4, 37°C Homo sapiens
0.127
-
 benzoyl-Gln-Arg CPMwt, pH 7.4, 37°C Homo sapiens
0.13
-
 benzoyl-Met-Arg CPMwt, pH 7.2, 37°C Homo sapiens
0.137
-
 benzoyl-Met-Arg hCPM, pH 7.2, 37°C Homo sapiens
0.138
-
 benzoyl-Met-Arg CPMwt, pH 7.4, 37°C Homo sapiens
0.162
-
 benzoyl-Ala-Arg CPMwt, pH 7.2, 37°C Homo sapiens
0.168
-
 benzoyl-Leu-Arg CPMwt, pH 7.4, 37°C Homo sapiens
0.18
-
 benzoyl-Ala-Arg hCPM, pH 7.2, 37°C Homo sapiens
0.192
-
 benzoyl-Thr-Arg CPMwt, pH 7.4, 37°C Homo sapiens
0.205
-
 benzoyl-Ile-Arg CPMwt, pH 7.4, 37°C Homo sapiens
0.206
-
 N-(4-hydroxybenzoyl)-Gly-Arg CPMwt, pH 7.2, 37°C Homo sapiens
0.219
-
 benzoyl-Ser-Arg CPMwt, pH 7.4, 37°C Homo sapiens
0.239
-
 benzoyl-His-Arg CPMwt, pH 7.4, 37°C Homo sapiens
0.241
-
 N-(4-hydroxybenzoyl)-Gly-Arg hCPM, pH 7.2, 37°C Homo sapiens
0.255
-
 benzoyl-Gly-Arg CPMwt, pH 7.2, 37°C Homo sapiens
0.264
-
 benzoyl-Val-Arg CPMwt, pH 7.4, 37°C Homo sapiens
0.289
-
 benzoyl-Gly-Arg CPMwt, pH 7.4, 37°C Homo sapiens
0.29
-
 benzoyl-Gly-Arg hCPM, pH 7.2, 37°C Homo sapiens
0.343
-
 benzoyl-Asn-Arg CPMwt, pH 7.4, 37°C Homo sapiens
1.152
-
 furylacryloyl-Ala-Lys CPMwt, pH 7.2, 37°C Homo sapiens
1.181
-
 Gly-Gly-Arg CPMwt, pH 7.2, 37°C Homo sapiens
10.15
-
 Benzoyl-Gly-Lys hCPM, pH 7.2, 37°C Homo sapiens
10.65
-
 Benzoyl-Gly-Lys CPMwt, pH 7.2, 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Homo sapiens 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 determined by SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P14384
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information hCPM and CPMwt differ in the nature and relative amount of the N-glycan carbohydrate moities Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
wild type carboxypeptidase M, CPMwt, is purified from Pichia pastoris supernatant using hydrophobic interaction chromatography and affinity chromatography, hCPM is purified from human prostasomes Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
 Homo sapiens
-
prostasome
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14
-
 CPMwt with dansyl-Ala-Arg Homo sapiens
19
-
 hCPM with dansyl-Ala-Arg Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ser-His-Leu-Gly-Leu-Ala-Arg + H2O human anaphylatoxin C3A fragment 70-77 Homo sapiens Ala-Ser-His-Leu-Gly-Leu-Ala + Arg
-
 ?
benzoyl-Ala-Arg + H2O
-
 Homo sapiens benzoyl-Ala + Arg
-
 ?
benzoyl-Asn-Arg + H2O
-
 Homo sapiens benzoyl-Asn + Arg
-
 ?
benzoyl-Gln-Arg + H2O
-
 Homo sapiens benzoyl-Gln + Arg
-
 ?
benzoyl-Gly-Arg + H2O
-
 Homo sapiens benzoyl-Gly + Arg
-
 ?
benzoyl-Gly-argininic acid + H2O
-
 Homo sapiens ?
-
 ?
benzoyl-Gly-Lys + H2O
-
 Homo sapiens benzoyl-Gly + Lys
-
 ?
benzoyl-His-Arg + H2O
-
 Homo sapiens benzoyl-His + Arg
-
 ?
benzoyl-Ile-Arg + H2O
-
 Homo sapiens benzoyl-Ile + Arg
-
 ?
benzoyl-Leu-Arg + H2O
-
 Homo sapiens benzoyl-Leu + Arg
-
 ?
benzoyl-Met-Arg + H2O
-
 Homo sapiens benzoyl-Met + Arg
-
 ?
benzoyl-Phe-Arg + H2O
-
 Homo sapiens benzoyl-Phe + Arg
-
 ?
benzoyl-Ser-Arg + H2O
-
 Homo sapiens benzoyl-Ser + Arg
-
 ?
benzoyl-Thr-Arg + H2O
-
 Homo sapiens benzoyl-Thr + Arg
-
 ?
benzoyl-Trp-Arg + H2O
-
 Homo sapiens benzoyl-Trp + Arg
-
 ?
benzoyl-Tyr-Arg + H2O
-
 Homo sapiens benzoyl-Tyr + Arg
-
 ?
benzoyl-Val-Arg + H2O
-
 Homo sapiens benzoyl-Val + Arg
-
 ?
bradykinin(-Phe-Arg) + H2O
-
 Homo sapiens ?
-
 ?
Dansyl-Ala-Arg + H2O enzyme assay Homo sapiens Dansyl-Ala + Arg
-
 ?
furylacryloyl-Ala-Arg + H2O
-
 Homo sapiens furylacryloyl-Ala + Arg
-
 ?
furylacryloyl-Ala-Lys + H2O
-
 Homo sapiens furylacryloyl-Ala + Lys
-
 ?
Gly-Gly-Arg + H2O
-
 Homo sapiens Gly-Gly + Arg
-
 ?
Gly-Leu-Ala-Arg + H2O human anaphylatoxin C3A fragment 74-77 Homo sapiens Gly-Leu-Ala + Arg
-
 ?
His-Leu-Gly-Leu-Ala-Arg + H2O human anaphylatoxin C3A fragment 72-77 Homo sapiens His-Leu-Gly-Leu-Ala + Arg
-
 ?
Leu-Ala-Arg + H2O human anaphylatoxin C3A fragment 75-77 Homo sapiens Leu-Ala + Arg
-
 ?
Leu-Gly-Leu-Ala-Arg + H2O human anaphylatoxin C3A fragment 73-77 Homo sapiens Leu-Gly-Leu-Ala + Arg
-
 ?
N-(4-hydroxybenzoyl)-Gly-Arg + H2O
-
 Homo sapiens N-(4-hydroxybenzoyl)-Gly + L-arginine
-
 ?
Pro-Gly-Lys-Ala-Arg + H2O
-
 Homo sapiens Pro-Gly-Lys-Ala + Arg
-
 ?
Ser-His-Leu-Gly-Leu-Ala-Arg + H2O human anaphylatoxin C3A fragment 71-77 Homo sapiens Ser-His-Leu-Gly-Leu-Ala + Arg
-
 ?

Synonyms

Synonyms Comment Organism
carboxypeptidase M
-
 Homo sapiens
CPM
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 enzyme assay Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
 benzoyl-Ile-Arg CPMwt, pH 7.4, 37°C Homo sapiens
1.9
-
 Gly-Gly-Arg CPMwt, pH 7.2, 37°C Homo sapiens
3.9
-
 benzoyl-Gly-Arg CPMwt, pH 7.4, 37°C Homo sapiens
4.2
-
 Leu-Ala-Arg CPMwt, pH 7.4, 37°C Homo sapiens
4.9
-
 N-(4-hydroxybenzoyl)-Gly-Arg CPMwt, pH 7.2, 37°C Homo sapiens
5.7
-
 benzoyl-Gly-Arg CPMwt, pH 7.2, 37°C Homo sapiens
5.7
-
 N-(4-hydroxybenzoyl)-Gly-Arg hCPM, pH 7.2, 37°C Homo sapiens
6.07
-
 benzoyl-Gly-Arg hCPM, pH 7.2, 37°C Homo sapiens
6.1
-
 Leu-Gly-Leu-Ala-Arg CPMwt, pH 7.4, 37°C Homo sapiens
6.6
-
 His-Leu-Gly-Leu-Ala-Arg CPMwt, pH 7.4, 37°C Homo sapiens
6.7
-
 Ser-His-Leu-Gly-Leu-Ala-Arg CPMwt, pH 7.4, 37°C Homo sapiens
7.4
-
 Ala-Ser-His-Leu-Gly-Leu-Ala-Arg CPMwt, pH 7.4, 37°C Homo sapiens
7.7
-
 benzoyl-Val-Arg CPMwt, pH 7.4, 37°C Homo sapiens
8.2
-
 Gly-Leu-Ala-Arg CPMwt, pH 7.4, 37°C Homo sapiens
9.6
-
 Benzoyl-Gly-Lys hCPM, pH 7.2, 37°C Homo sapiens
11
-
 Benzoyl-Gly-Lys CPMwt, pH 7.2, 37°C Homo sapiens
11
-
 furylacryloyl-Ala-Lys CPMwt, pH 7.2, 37°C Homo sapiens
11
-
 Pro-Gly-Lys-Ala-Arg CPMwt, pH 7.2, 37°C Homo sapiens
14
-
 benzoyl-His-Arg CPMwt, pH 7.4, 37°C Homo sapiens
17
-
 Dansyl-Ala-Arg CPMwt, pH 7.2, 37°C Homo sapiens
17.6
-
 Dansyl-Ala-Arg hCPM, pH 7.2, 37°C Homo sapiens
19
-
 benzoyl-Leu-Arg CPMwt, pH 7.4, 37°C Homo sapiens
22
-
 benzoyl-Gln-Arg CPMwt, pH 7.4, 37°C Homo sapiens
22.9
-
 benzoyl-Thr-Arg CPMwt, pH 7.4, 37°C Homo sapiens
23
-
 benzoyl-Trp-Arg CPMwt, pH 7.4, 37°C Homo sapiens
27
-
 benzoyl-Tyr-Arg CPMwt, pH 7.4, 37°C Homo sapiens
30
-
 bradykinin(-Phe-Arg) CPMwt, pH 7.2, 37°C Homo sapiens
32
-
 furylacryloyl-Ala-Arg hCPM, pH 7.2, 37°C Homo sapiens
33
-
 benzoyl-Phe-Arg CPMwt, pH 7.4, 37°C Homo sapiens
35
-
 benzoyl-Asn-Arg CPMwt, pH 7.4, 37°C Homo sapiens
40
-
 furylacryloyl-Ala-Arg CPMwt, pH 7.2, 37°C Homo sapiens
60
-
 benzoyl-Ser-Arg CPMwt, pH 7.4, 37°C Homo sapiens
77
-
 benzoyl-Ala-Arg CPMwt, pH 7.4, 37°C Homo sapiens
87
-
 benzoyl-Met-Arg CPMwt, pH 7.4, 37°C Homo sapiens
88
-
 benzoyl-Ala-Arg hCPM, pH 7.2, 37°C Homo sapiens
107
-
 benzoyl-Ala-Arg CPMwt, pH 7.2, 37°C Homo sapiens
112
-
 benzoyl-Met-Arg hCPM, pH 7.2, 37°C Homo sapiens
130
-
 benzoyl-Met-Arg CPMwt, pH 7.2, 37°C Homo sapiens
273
-
 benzoyl-Gly-argininic acid hCPM, pH 7.2, 37°C Homo sapiens
316
-
 benzoyl-Gly-argininic acid CPMwt, pH 7.2, 37°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 enzyme assay Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000003
-
 MERGETPA CPMwt Homo sapiens
0.000006
-
 MERGETPA hCPM Homo sapiens