Cloned (Comment) | Organism |
---|---|
expression of 3'-truncated, glycosylphosphatidylinositol-free, soluble enzyme in insect cells using the baculovirus transfection system | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified, recombinant, glycosylphosphatidylinositol-free enzyme, X-ray diffraction structure determination and analysis at 3.0 A resolution, modelling | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell surface | - |
Homo sapiens | 9986 | - |
extracellular | - |
Homo sapiens | - |
- |
plasma membrane | anchored via glycosylphosphatidylinositol | Homo sapiens | 5886 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
1 * 50000, recombinant 3-truncated, glycosylphosphatidylinositol-free enzyme, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the enzyme regulates peptide hormones and growth factors at the cell surface, and is active in the degradation of extracellular proteins in the membrane | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P14384 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | N-glycosylation at Asn89 and partially at Asn21 | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant 3'-truncated, glycosylphosphatidylinositol-free, soluble enzyme from insect cells by anion exchange chromatography and gel filtration to homogeneity | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
cleavage of C-terminal arginine or lysine residues from polypeptides | catalytic mechanism, active site groove structure involving residues Y242, W241, K228, K269, E264, R127, H66, E69, and H173, substrate interactins | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | wide tissue distribution | Homo sapiens | - |
placenta | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme regulates peptide hormones and growth factors at the cell surface, and is active in the degradation of extracellular proteins in the membrane | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, recombinant 3'-truncated, glycosylphosphatidylinositol-free enzyme, SDS-PAGE | Homo sapiens |
More | catalytic domain structure | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
CPM | - |
Homo sapiens |