Application | Comment | Organism |
---|---|---|
medicine | VanX, being a required enzyme for bacterial antibiotic resistance, should be a key target in circumventing clinical vancomycin resistance | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
into the pMAL-c2X vector for expression in Escherichia coli JM109 cells | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-Ala(P,O)D-Ala | acts as slow binding inhibitor | Escherichia coli | |
D-Ala(P,O)D-Phe | acts as slow binding inhibitor | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | - |
Escherichia coli | 5618 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
determined by SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Ala-D-Ala + H2O | Escherichia coli | - |
D-Ala + D-Ala | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using an amylose affinity column, the MBP-tag is removed by factor Xa cleavage | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Ala-D-Ala + H2O | - |
Escherichia coli | D-Ala + D-Ala | - |
? | |
D-Ala-D-Phe + H2O | - |
Escherichia coli | D-Ala + D-Phe | - |
? | |
D-Ala-D-Trp + H2O | - |
Escherichia coli | D-Ala + D-Trp | - |
? | |
D-Ala-D-Tyr + H2O | - |
Escherichia coli | D-Ala + D-Tyr | - |
? |
Synonyms | Comment | Organism |
---|---|---|
D,D-dipeptidase | - |
Escherichia coli |
serine-type D-Ala-D-Ala carboxypeptidase | - |
Escherichia coli |
VanX | - |
Escherichia coli |