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Literature summary for 3.4.16.4 extracted from

  • Chang, Y.P.; Tseng, M.J.; Chu, Y.H.
    Using surface plasmon resonance to directly measure slow binding of low-molecular mass inhibitors to a VanX chip (2006), Anal. Biochem., 359, 63-71.
    View publication on PubMed

Application

Application Comment Organism
medicine VanX, being a required enzyme for bacterial antibiotic resistance, should be a key target in circumventing clinical vancomycin resistance Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
into the pMAL-c2X vector for expression in Escherichia coli JM109 cells Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
D-Ala(P,O)D-Ala acts as slow binding inhibitor Escherichia coli
D-Ala(P,O)D-Phe acts as slow binding inhibitor Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall
-
Escherichia coli 5618
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+
-
Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
determined by SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-Ala-D-Ala + H2O Escherichia coli
-
D-Ala + D-Ala
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using an amylose affinity column, the MBP-tag is removed by factor Xa cleavage Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Ala-D-Ala + H2O
-
Escherichia coli D-Ala + D-Ala
-
?
D-Ala-D-Phe + H2O
-
Escherichia coli D-Ala + D-Phe
-
?
D-Ala-D-Trp + H2O
-
Escherichia coli D-Ala + D-Trp
-
?
D-Ala-D-Tyr + H2O
-
Escherichia coli D-Ala + D-Tyr
-
?

Synonyms

Synonyms Comment Organism
D,D-dipeptidase
-
Escherichia coli
serine-type D-Ala-D-Ala carboxypeptidase
-
Escherichia coli
VanX
-
Escherichia coli