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Literature summary for 3.4.16.4 extracted from

  • Leyh-Bouille, M.; Nguyen-Disteche, M.; Ghuysen, J.M.
    On the DD-carboxypeptidase enzyme system of Streptomyces strain K15 (1981), Eur. J. Biochem., 115, 579-584.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
benzylpenicillin
-
Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
Nalpha,Nepsilon-Diacetyl-L-Lys-D-Ala-D-Ala 54000 MW enzyme Streptomyces sp.
12
-
Nalpha, Nepsilon-Diacetyl-L-Lys-D-Ala-D-Ala lysozyme releasable enzyme Streptomyces sp.
14
-
Nalpha,Nepsilon-Diacetyl-L-Lys-D-Ala-D-Ala
-
Streptomyces sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Streptomyces sp.
-
-
membrane lysozyme releasable Streptomyces sp. 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
microdensitometry Streptomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-muramyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine Streptomyces sp.
-
?
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
sp. K15
-

Purification (Commentary)

Purification (Comment) Organism
partial Streptomyces sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + Gly-Gly acceptors in transpeptidase reaction Streptomyces sp. Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-Gly-Gly + D-Ala
-
?
UDP-N-acetyl-muramyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine
-
Streptomyces sp. ?
-
?
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine + H2O
-
Streptomyces sp. UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanine + D-alanine
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
267
-
diacetyl-L-Lys-D-Ala-D-Ala 54000 MW enzyme Streptomyces sp.
350
-
diacetyl-L-Lys-D-Ala-D-Ala 40000 MW enzyme Streptomyces sp.