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Literature summary for 3.4.16.4 extracted from

  • Frere, J.M.; Joris, B.
    Penicillin-sensitive enzymes in peptidoglycan biosynthesis (1985), CRC Crit. Rev. Microbiol., 11, 299-396.
    View publication on PubMed
Search for more literature for 3.4.16.4

Inhibitors

Inhibitors Comment Organism Structure
penicillin kinetic model of interaction Actinomadura sp.
penicillin kinetic model of interaction Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 Nalpha,Nepsilon-Diacetyl-L-Lys-D-Ala-D-Ala 54000 MW enzyme Streptomyces sp.
12
-
 Nalpha, Nepsilon-Diacetyl-L-Lys-D-Ala-D-Ala lysozyme releasable enzyme Streptomyces sp.
14
-
 Nalpha,Nepsilon-Diacetyl-L-Lys-D-Ala-D-Ala
-
 Streptomyces sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Streptomyces sp.
-
-
extracellular
-
 Actinomadura sp.
-
-
membrane
-
 Streptomyces sp. 16020
-
membrane
-
 Actinomadura sp. 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 gel filtration + mercaptoethanol, SDS-PAGE Streptomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-muramyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine Streptomyces sp.
-
 ?
-
 ?
UDP-N-acetyl-muramyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine Actinomadura sp.
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Actinomadura sp.
-
 R39
-
Streptomyces sp.
-
 R61
-
Streptomyces sp.
-
 sp. K15
-

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Streptomyces sp.
-
culture filtrate
-
 Actinomadura sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + Gly-Gly acceptors in transpeptidase reaction Streptomyces sp. Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-Gly-Gly + D-Ala
-
 ir
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
-
 Streptomyces sp. Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-alanine
-
 ?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
-
 Actinomadura sp. Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-alanine
-
 ?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-lactate + H2O
-
 Streptomyces sp. Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-lactate
-
 ?
UDP-N-acetyl-muramyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine
-
 Streptomyces sp. ?
-
 ?
UDP-N-acetyl-muramyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine
-
 Actinomadura sp. ?
-
 ?
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine + H2O
-
 Streptomyces sp. UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanine + D-alanine
-
 ?
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine + H2O
-
 Actinomadura sp. UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanine + D-alanine
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 ratio of hydrolysis/transpeptidation depending on pH Streptomyces sp.
additional information
-
 ratio of hydrolysis/transpeptidation depending on pH Actinomadura sp.
6 8 hydrolysis, value increased by high ionic strength Streptomyces sp.
6 8 hydrolysis, value increased by high ionic strength Actinomadura sp.
8
-
 transpeptidation, Streptomyces sp. R61 Streptomyces sp.
9.5
-
 transpeptidation, Streptomyces sp. R39 Streptomyces sp.