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Literature summary for 3.4.16.2 extracted from

  • Goptar, I.A.; Shagin, D.A.; Shagina, I.A.; Mudrik, E.S.; Smirnova, Y.A.; Zhuzhikov, D.P.; Belozersky, M.A.; Dunaevsky, Y.E.; Oppert, B.; Filippova, I.Y.; Elpidina, E.N.
    A digestive prolyl carboxypeptidase in Tenebrio molitor larvae (2013), Insect Biochem. Mol. Biol., 43, 501-509.
    View publication on PubMed
Search for more literature for 3.4.16.2

Cloned(Commentary)

Cloned (Comment) Organism
-
 Tenebrio molitor

Inhibitors

Inhibitors Comment Organism Structure
N-benzyloxycarbonyl-Pro-prolinal
-
 Tenebrio molitor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.095
-
 N-benzyloxycarbonyl-L-Ala-L-Pro 4-nitroanilide pH 5.6, 30°C Tenebrio molitor
0.147
-
 N-benzyloxycarbonyl-Gly-L-Pro 4-nitroanilide pH 5.6, 30°C Tenebrio molitor
0.157
-
 L-Ala-L-Pro 4-nitroanilide pH 5.6, 30°C Tenebrio molitor
0.17
-
 N-benzyloxycarbonyl-L-Pro 4-nitroanilide pH 5.6, 30°C Tenebrio molitor
0.34
-
 N-benzyloxycarbonyl-L-Ala-L-Ala-L-Pro 4-nitroanilide pH 5.6, 30°C Tenebrio molitor

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53554
-
 2 * 53554, mass spectrometry of mature protein Tenebrio molitor
105000
-
 gel filtration Tenebrio molitor

Organism

Organism UniProt Comment Textmining
Tenebrio molitor C5H0E3
-
-

Source Tissue

Source Tissue Comment Organism Textmining
larva anterior midgut of larvae Tenebrio molitor
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gliadin + H2O
-
 Tenebrio molitor ? enzyme is able to cleave gliadins, the major dietary proteins of Tenebrio molitor larvae ?
L-Ala-L-Pro 4-nitroanilide + H2O
-
 Tenebrio molitor L-Ala-L-Pro + 4-nitroaniline
-
 ?
additional information no substrates: L-Pro 4-nitroanilide, succinyl-L-Ala-L-Ala-L-Pro-L-Phe 4-nitroanilide, succinyl-L-Ala-L-Ala-L-Pro-L-Leu 4-nitroanilide. Enzyme is strictly specific for proline residues Tenebrio molitor ?
-
 ?
N-benzyloxycarbonyl-Gly-L-Pro 4-nitroanilide + H2O
-
 Tenebrio molitor N-benzyloxycarbonyl-Gly-L-Pro + 4-nitroaniline
-
 ?
N-benzyloxycarbonyl-L-Ala-L-Ala-L-Pro 4-nitroanilide + H2O
-
 Tenebrio molitor N-benzyloxycarbonyl-L-Ala-L-Ala-L-Pro + 4-nitroaniline
-
 ?
N-benzyloxycarbonyl-L-Ala-L-Pro 4-nitroanilide + H2O
-
 Tenebrio molitor N-benzyloxycarbonyl-L-Ala-L-Pro + 4-nitroaniline
-
 ?
N-benzyloxycarbonyl-L-Pro 4-nitroanilide + H2O
-
 Tenebrio molitor N-benzyloxycarbonyl-L-Pro + 4-nitroaniline
-
 ?
N-benzyloxycarbonyl-L-Pro-L-Phe + H2O
-
 Tenebrio molitor N-benzyloxycarbonyl-L-Pro + L-Phe
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 53554, mass spectrometry of mature protein Tenebrio molitor

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0037
-
 N-benzyloxycarbonyl-Pro-prolinal pH 5.6, 30°C Tenebrio molitor

General Information

General Information Comment Organism
physiological function enzyme participates in the hydrolysis of the insect’s major dietary proteins, gliadins Tenebrio molitor