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Literature summary for 3.4.15.1 extracted from

  • Ogasawara, W.; Abe, N.; Hagio, T.; Okada, H.; Morikawa, Y.
    Purification and characterization of a dipeptidyl carboxypeptidase from Pseudomonas sp. WO24 (1997), Biosci. Biotechnol. Biochem., 61, 858-863.
    View publication on PubMed
Search for more literature for 3.4.15.1

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Pseudomonas sp.
CaCl2
-
 Pseudomonas sp.
CuCl2
-
 Pseudomonas sp.
EDTA
-
 Pseudomonas sp.
MgCl2
-
 Pseudomonas sp.
Mn2+ MnCl2 Pseudomonas sp.
NaCl 500 mM, 50% inhibition Pseudomonas sp.
PO43- 100 mM, 50% inhibition Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl 500 mM, 50% inhibition Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
 gel filtration Pseudomonas sp.
74000
-
 1 * 74000, SDS-PAGE Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 WO24
-
Pseudomonas sp. WO24
-
 WO24
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
780
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-Ala-Ala + H2O
-
 Pseudomonas sp. Ala-Ala + Ala-Ala
-
 ?
Ala-Ala-Ala-Ala + H2O
-
 Pseudomonas sp. WO24 Ala-Ala + Ala-Ala
-
 ?
angiotensin I + H2O
-
 Pseudomonas sp. angiotensin II + His-Leu
-
 ?
angiotensin I + H2O
-
 Pseudomonas sp. WO24 angiotensin II + His-Leu
-
 ?
benzoyl-Gly-Ala-Pro + H2O
-
 Pseudomonas sp. benzoyl-Gly + Ala-Pro
-
 ?
benzoyl-Gly-Ala-Pro + H2O
-
 Pseudomonas sp. WO24 benzoyl-Gly + Ala-Pro
-
 ?
benzoyl-Gly-His-Leu + H2O
-
 Pseudomonas sp. benzoyl-Gly + His-Leu
-
 ?
benzoyl-Gly-His-Leu + H2O
-
 Pseudomonas sp. WO24 benzoyl-Gly + His-Leu
-
 ?
benzyloxycarbonyl-(NO2)Phe-His-Leu + H2O
-
 Pseudomonas sp. benzyloxycarbonyl-(NO2)Phe + His-Leu
-
 ?
benzyloxycarbonyl-Phe-Tyr-Leu
-
 Pseudomonas sp. benzyloxycarbonyl-Phe + Tyr-Leu
-
 ?
bradykinin + H2O
-
 Pseudomonas sp. Phe-Arg + Ser-Pro + Arg-Pro-Pro-Gly-Phe hydrolyzes Phe-Arg + Ser-Pro from the C-terminus of bradykinin ?
bradykinin + H2O
-
 Pseudomonas sp. WO24 Phe-Arg + Ser-Pro + Arg-Pro-Pro-Gly-Phe hydrolyzes Phe-Arg + Ser-Pro from the C-terminus of bradykinin ?

Subunits

Subunits Comment Organism
monomer 1 * 74000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 stable for at least 30 min Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.5 12 no activity below pH 5.5 and above pH 12.0 Pseudomonas sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
100
-
 PO43-
-
 Pseudomonas sp.
500
-
 NaCl
-
 Pseudomonas sp.