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Literature summary for 3.4.14.9 extracted from

  • Oyama, H.; Fujisawa, T.; Suzuki, T.; Dunn, B.M.; Wlodawer, A.; Oda, K.
    Catalytic residues and substrate specificity of recombinant human tripeptidyl peptidase I (CLN2) (2005), J. Biochem., 138, 127-134.
    View publication on PubMed
Search for more literature for 3.4.14.9

Cloned(Commentary)

Cloned (Comment) Organism
expression in silkworm pupae Homo sapiens

Protein Variants

Protein Variants Comment Organism
D165A inactive mutant Homo sapiens
D81A not expressed in Sf9 cells Homo sapiens
E77A very low activity with Ala-Arg-Phe-p-nitrophenylalanyl-Arg-Leu Homo sapiens
S280A inactive mutant Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
acetylpepstatin 1 mM, 14% inhibition Homo sapiens
Ala-Ala-Phe-chloromethylketone 0.1 mM, complete inhibition Homo sapiens
chymostatin 1 mM, 47% inhibition Homo sapiens
N-acetyl-Ile-Ala-phenylalaninal 1 mM, 88% inhibition Homo sapiens
N-acetyl-Ile-Pro-phenylalaninal 1 mM, 38% inhibition Homo sapiens
Tyrostatin 1 mM, 19% inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00402
-
 Ala-Arg-Phe-p-nitrophenylalanyl-Arg-Leu pH 4.0, 37°C, wild-type enzyme Homo sapiens
0.00509
-
 Ala-Asp-Phe-p-nitrophenylalanyl-Arg-Leu pH 4.0, 37°C, wild-type enzyme Homo sapiens
0.0148
-
 Ala-Ala-Phe-p-nitrophenylalanyl-Arg-Leu pH 4.0, 37°C, wild-type enzyme Homo sapiens
0.0193
-
 Arg-Ala-Phe-p-nitrophenylalanyl-Arg-Leu pH 4.0, 37°C, wild-type enzyme Homo sapiens
0.0422
-
 Ala-His-Phe-p-nitrophenylalanyl-Arg-Leu pH 4.0, 37°C, wild-type enzyme Homo sapiens
0.0483
-
 Ala-Ser-Phe-p-nitrophenylalanyl-Arg-Leu pH 4.0, 37°C, wild-type enzyme Homo sapiens
0.108
-
 Ser-Ala-Phe-p-nitrophenylalanyl-Arg-Leu pH 4.0, 37°C, wild-type enzyme Homo sapiens
0.11
-
 Asp-Ala-Phe-p-nitrophenylalanyl-Arg-Leu pH 4.0, 37°C, wild-type enzyme Homo sapiens
0.123
-
 Ala-Arg-Phe-p-nitrophenylalanyl-Arg-Leu pH 4.0, 37°C, mutant enzyme E77A Homo sapiens
0.167
-
 His-Ala-Phe-p-nitrophenylalanyl-Arg-Leu pH 4.0, 37°C, wild-type enzyme Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 x * 46000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification TPP-I is synthesized as a 66000 Da inactive precursor consisting of a 195 residue prepro-peptide and 368 residue mature enzyme. Most of the precursor proteins are converted to a 46000 Da form by post-translational proteolysis Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
 ?
Ala-Ala-Phe-p-nitrophenylalanyl-Arg-Leu + H2O
-
 Homo sapiens Ala-Ala-Phe + p-nitrophenylalanyl-Arg-Leu
-
 ?
Ala-Arg-Phe-p-nitrophenylalanyl-Arg-Leu + H2O
-
 Homo sapiens Ala-Arg-Phe + p-nitrophenylalanyl-Arg-Leu
-
 ?
Ala-Asp-Phe-p-nitrophenylalanyl-Arg-Leu + H2O
-
 Homo sapiens Ala-Asp-Phe + p-nitrophenylalanyl-Arg-Leu
-
 ?
Ala-His-Phe-p-nitrophenylalanyl-Arg-Leu + H2O
-
 Homo sapiens Ala-His-Phe + p-nitrophenylalanyl-Arg-Leu
-
 ?
Ala-Ser-Phe-p-nitrophenylalanyl-Arg-Leu + H2O
-
 Homo sapiens Ala-Ser-Phe + p-nitrophenylalanyl-Arg-Leu
-
 ?
Arg-Ala-Phe-p-nitrophenylalanyl-Arg-Leu + H2O
-
 Homo sapiens Arg-Ala-Phe + p-nitrophenylalanyl-Arg-Leu
-
 ?
Asp-Ala-Phe-p-nitrophenylalanyl-Arg-Leu + H2O
-
 Homo sapiens Asp-Ala-Phe + p-nitrophenylalanyl-Arg-Leu
-
 ?
His-Ala-Phe-p-nitrophenylalanyl-Arg-Leu + H2O
-
 Homo sapiens His-Ala-Phe + p-nitrophenylalanyl-Arg-Leu
-
 ?
Ser-Ala-Phe-p-nitrophenylalanyl-Arg-Leu + H2O
-
 Homo sapiens Ser-Ala-Phe + p-nitrophenylalanyl-Arg-Leu
-
 ?

Subunits

Subunits Comment Organism
? x * 46000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
CLN2
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0124
-
 Ala-Arg-Phe-p-nitrophenylalanyl-Arg-Leu pH 4.0, 378°C, mutant enzyme E77A Homo sapiens
0.91
-
 Arg-Ala-Phe-p-nitrophenylalanyl-Arg-Leu pH 4.0, 37°C, wild-type enzyme Homo sapiens
4.7
-
 Ser-Ala-Phe-p-nitrophenylalanyl-Arg-Leu pH 4.0, 37°C, wild-type enzyme Homo sapiens
11
-
 Ala-Asp-Phe-p-nitrophenylalanyl-Arg-Leu pH 4.0, 37°C, wild-type enzyme Homo sapiens
11.8
-
 Ala-Arg-Phe-p-nitrophenylalanyl-Arg-Leu pH 4.0, 37°C, wild-type enzyme Homo sapiens
13.7
-
 His-Ala-Phe-p-nitrophenylalanyl-Arg-Leu pH 4.0, 37°C, wild-type enzyme Homo sapiens
18.5
-
 Asp-Ala-Phe-p-nitrophenylalanyl-Arg-Leu pH 4.0, 37°C, wild-type enzyme Homo sapiens
26.4
-
 Ala-His-Phe-p-nitrophenylalanyl-Arg-Leu pH 4.0, 37°C, wild-type enzyme Homo sapiens
30.7
-
 Ala-Ser-Phe-p-nitrophenylalanyl-Arg-Leu pH 4.0, 37°C, wild-type enzyme Homo sapiens
34.5
-
 Ala-Ala-Phe-p-nitrophenylalanyl-Arg-Leu pH 4.0, 37°C, wild-type enzyme Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
3 4.8 pH 3.0: about 30% of maximal activity, pH 4.8: about 50% of maximal activity Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.5 5 stable Homo sapiens