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Literature summary for 3.4.14.9 extracted from

  • Reichard, U.; Lechenne, B.; Asif, A.R.; Streit, F.; Grouzmann, E.; Jousson, O.; Monod, M.
    Sedolisins, a new class of secreted proteases from Aspergillus fumigatus with endoprotease or tripeptidyl-peptidase activity at acidic pHs (2006), Appl. Environ. Microbiol., 72, 1739-1748.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.14.9

Cloned(Commentary)

Cloned (Comment) Organism
expression of SedB in Pichia pastoris Aspergillus fumigatus
expression of SedC in Pichia pastoris Aspergillus fumigatus
expression of SedD in Pichia pastoris Aspergillus fumigatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0625
-
 Phe-Pro-Ala-4-nitroanilide pH 6.0, 20°C, SedB Aspergillus fumigatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40600
-
 x * 40600, SedD, calculated from sequence Aspergillus fumigatus
43000
-
 x * 43000, SedB, calculated from sequence Aspergillus fumigatus

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus Q70GH4 FuSed3 protease precursor
-
Aspergillus fumigatus Q70J58 FuSed4 protease precursor
-
Aspergillus fumigatus Q70J59 FuSed2 protease precursor
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the preproprotein SedB is composed of 602 amino acids, the signal peptide contains 24 amino acids Aspergillus fumigatus
proteolytic modification the preproprotein SedC is composed of 596 amino acids, the signal peptide contains 24 amino acids Aspergillus fumigatus
proteolytic modification the preproprotein SedD is composed of 568 amino acids, the signal peptide contains 20 amino acids Aspergillus fumigatus

Purification (Commentary)

Purification (Comment) Organism
recombinant SedB Aspergillus fumigatus
recombinant SedD Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-Phe-4-nitroanilide + H2O SedB Aspergillus fumigatus Ala-Ala-Phe + 4-nitroaniline
-
 ?
Ala-Ala-Phe-4-nitroanilide + H2O SedC Aspergillus fumigatus Ala-Ala-Phe + 4-nitroaniline
-
 ?
Ala-Ala-Phe-4-nitroanilide + H2O SedD Aspergillus fumigatus Ala-Ala-Phe + 4-nitroaniline
-
 ?
Ala-Pro-Gly-Asp-Arg-Ile-Tyr-Val-His-Pro-Phe + H2O SedB Aspergillus fumigatus Ala-Pro-Gly + Asp-Arg-Ile + Tyr-Val-His-Pro-Phe
-
 ?
Phe-Pro-Ala-4-nitroanilide + H2O SedB Aspergillus fumigatus Phe-Pro-Ala + 4-nitroaniline
-
 ?
Phe-Pro-Ala-4-nitroanilide + H2O SedC Aspergillus fumigatus Phe-Pro-Ala + 4-nitroaniline
-
 ?
Phe-Pro-Ala-4-nitroanilide + H2O SedD Aspergillus fumigatus Phe-Pro-Ala + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 40600, SedD, calculated from sequence Aspergillus fumigatus
? x * 43000, SedB, calculated from sequence Aspergillus fumigatus

Synonyms

Synonyms Comment Organism
SedB
-
 Aspergillus fumigatus
SedC
-
 Aspergillus fumigatus
sedolisin B
-
 Aspergillus fumigatus
sedolisin C
-
 Aspergillus fumigatus
sedolisin D
-
 Aspergillus fumigatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
35
-
 Phe-Pro-Ala-4-nitroanilide pH 6.0, 20°C, SedB Aspergillus fumigatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 SedD Aspergillus fumigatus
6
-
 SedB Aspergillus fumigatus
6
-
 SedC Aspergillus fumigatus

pH Range

pH Minimum pH Maximum Comment Organism
1.5 6 active between pH 3 and pH 7, SedD Aspergillus fumigatus
3 7 active between pH 3 and pH 7, SedB Aspergillus fumigatus
3 7 active between pH 3 and pH 7, SedC Aspergillus fumigatus

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus fumigatus calculated from sequence, mature domain, SedD
-
 5.12
Aspergillus fumigatus calculated from sequence, mature domain, SedB
-
 5.17