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Literature summary for 3.4.14.9 extracted from

  • Page, A.E.; Fuller, K.; Chambers, T.J.; Warburton, M.J.
    Purification and characterization of a tripeptidyl peptidase I from human osteoclastomas: evidence for its role in bone resorption (1993), Arch. Biochem. Biophys., 306, 354-359.
    View publication on PubMed
Search for more literature for 3.4.14.9

Inhibitors

Inhibitors Comment Organism Structure
3,4-dichloroisocoumarin weak Homo sapiens
diethyldicarbonate
-
 Homo sapiens
diisopropylfluorophosphate at high concentration Homo sapiens
additional information no inhibition by pepstatin; no inhibition: EDTA; no inhibition: Phe-Pro-Arg chloromethylketone, inhibitors of cysteine proteinases or metalloproteinases, iodoacetamide, trans-epoxysuccinyl-L-leucinamido(4-guanidino)butane (i.e. E-64), mercaptoethanol, bestatin, soybean trypsin inhibitor, phenylchloromethyl ketone Homo sapiens
p-tosyl-L-Lys chloromethyl ketone weak Homo sapiens
Phenyl-N-phenylphosphoamidochloridate weak Homo sapiens
phenylmethylsulfonyl fluoride weak Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Homo sapiens 5764
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 x * 48000, SDS-PAGE Homo sapiens
700000
-
 gel filtration in the absence or presence of 4 M urea Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens involved in degradation of bone collagen ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
osteoclastoma cell bone tumor Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
 ?
Gly-Pro-Met 2-naphthylamide + H2O
-
 Homo sapiens Gly-Pro-Met + 2-naphthylamine
-
 ?
additional information succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin, succinyl-Gly-Pro-Leu 4-methylcoumarin 7-amide, Gly-Pro-7-amido-4-methylcoumarin, Gly-7-amido-4-methylcoumarin, Pro-7-amido-4-methylcoumarin, Met-7-amido-4-methylcoumarin, Ala 7-amido-4-methylcoumarin, Phe-7-amido-4-methylcoumarin, or Leu-7-amido-4-methylcoumarin Homo sapiens ?
-
 ?
additional information absolute requirement for unsubstituted amino-terminus Homo sapiens ?
-
 ?
additional information involved in degradation of bone collagen Homo sapiens ?
-
 ?
synthetic collagen-like polypetides + H2O
-
 Homo sapiens Gly-Pro-Xaa tripeptides
-
 ?

Subunits

Subunits Comment Organism
? x * 48000, SDS-PAGE Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 5.5 hydrolysis of Gly-Pro-Met 2-naphthylamide or Ala-Ala-Phe 4-methylcoumarin 7-amide Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
3 8 about half-maximal activity at pH 3 and 8, hydrolysis of Gly-Pro-Met 2-naphthylamide Homo sapiens
3.5 6.5 about half-maximal activity at pH 3.5 and 6.5, hydrolysis of Ala-Ala-Phe 4-methylcoumarin 7-amide Homo sapiens