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Literature summary for 3.4.14.5 extracted from

  • Rasmussen, H.B.; Branner, S.; Wiberg, F.C.; Wagtmann, N.
    Crystal structure of human dipeptidyl peptidase IV/CD26 in complex with a substrate analog (2003), Nat. Struct. Biol., 10, 19-25.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of a soluble enzyme form, which comprises amino acid residues 39-766 and is secreted to the medium, in Hi5 insect cells via baculovirus infection Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
40 mg/ml extracellular enzyme region complexed with inhibitor valine-pyrrolidine in 20 mM Tris, pH 8.0, ans 25 mM NaCl, sitting drop vapour diffusion method, mixed with equal volume of reservoir solution containing 0.3 M sodium acetate, 17-18% w/v PEG 4000, 0.1 M Tris, pH 8.0, 2-3 days, X-ray diffraction structure determination and analysis at 2.5 A resolution Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information alpha/beta-hydrolase domain and beta-propeller domain both participate in inhibitor binding Homo sapiens
valine-pyrrolidine
-
Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane type II transmembrane enzyme Homo sapiens 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
84400
-
non-glycosylated enzyme, calculated from amino acid sequence Homo sapiens
93500
-
recombinant soluble enzyme, MALDI mass spectrometry Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens contributes to the regulation of various physiological processes, including blood sugar homeostasis, by cleaving peptide hormones, chemokines, and neuropeptides ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P27487 multifunctional enzyme
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 11% glycosylation Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant soluble enzyme from insect cell culture supernatant to homogeneity Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
release of an N-terminal dipeptide, Xaa-Yaa-/-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline enzyme is a serine protease, catalytic and substrate binding mechanism, active site structure, catalytic triad Ser630, Asp708, His740 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly-Pro-4-nitroanilide + H2O
-
Homo sapiens Gly-Pro + 4-nitroaniline
-
?
additional information contributes to the regulation of various physiological processes, including blood sugar homeostasis, by cleaving peptide hormones, chemokines, and neuropeptides Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
dimer homodimer, crystal structure Homo sapiens
More the catalytic site is located in a large cavity formed between the alpha/beta-hydrolase domain and an eight-bladed beta-propeller domain Homo sapiens

Synonyms

Synonyms Comment Organism
CD26
-
Homo sapiens
dipeptidyl peptidase IV
-
Homo sapiens
DPP-IV
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens