Cloned (Comment) | Organism |
---|---|
expression of a soluble enzyme form, which comprises amino acid residues 39-766 and is secreted to the medium, in Hi5 insect cells via baculovirus infection | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
40 mg/ml extracellular enzyme region complexed with inhibitor valine-pyrrolidine in 20 mM Tris, pH 8.0, ans 25 mM NaCl, sitting drop vapour diffusion method, mixed with equal volume of reservoir solution containing 0.3 M sodium acetate, 17-18% w/v PEG 4000, 0.1 M Tris, pH 8.0, 2-3 days, X-ray diffraction structure determination and analysis at 2.5 A resolution | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | alpha/beta-hydrolase domain and beta-propeller domain both participate in inhibitor binding | Homo sapiens | |
valine-pyrrolidine | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | type II transmembrane enzyme | Homo sapiens | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
84400 | - |
non-glycosylated enzyme, calculated from amino acid sequence | Homo sapiens |
93500 | - |
recombinant soluble enzyme, MALDI mass spectrometry | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | contributes to the regulation of various physiological processes, including blood sugar homeostasis, by cleaving peptide hormones, chemokines, and neuropeptides | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P27487 | multifunctional enzyme | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 11% glycosylation | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant soluble enzyme from insect cell culture supernatant to homogeneity | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
release of an N-terminal dipeptide, Xaa-Yaa-/-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline | enzyme is a serine protease, catalytic and substrate binding mechanism, active site structure, catalytic triad Ser630, Asp708, His740 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gly-Pro-4-nitroanilide + H2O | - |
Homo sapiens | Gly-Pro + 4-nitroaniline | - |
? | |
additional information | contributes to the regulation of various physiological processes, including blood sugar homeostasis, by cleaving peptide hormones, chemokines, and neuropeptides | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | homodimer, crystal structure | Homo sapiens |
More | the catalytic site is located in a large cavity formed between the alpha/beta-hydrolase domain and an eight-bladed beta-propeller domain | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
CD26 | - |
Homo sapiens |
dipeptidyl peptidase IV | - |
Homo sapiens |
DPP-IV | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |