Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | not affected by dithiothreitol | Porphyromonas gingivalis |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli | Porphyromonas gingivalis |
Protein Variants | Comment | Organism |
---|---|---|
D702A | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Porphyromonas gingivalis |
H743A | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Porphyromonas gingivalis |
additional information | compared to the wild-type strain, an enzyme-deficient mutant strain leads to less severe infections and a faster recovery from the infection when injected into mice | Porphyromonas gingivalis |
S624A | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Porphyromonas gingivalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not affected by dithiothreitol | Porphyromonas gingivalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
Gly-Pro-4-nitroanilide | recombinant enzyme, pH 7.5, 25°C | Porphyromonas gingivalis | |
0.28 | - |
Gly-Pro-4-nitroanilide | native enzyme, pH 7.5, 25°C | Porphyromonas gingivalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74000 | - |
2 * 74000, native enzyme, SDS-PAGE | Porphyromonas gingivalis |
140000 | - |
native enzyme, gel filtration | Porphyromonas gingivalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Porphyromonas gingivalis | O66223 | periodontal pathogen, strain W38, gene dpp | - |
Purification (Comment) | Organism |
---|---|
native enzyme 1000fold to homogeneity, and recombinant enzyme from Escherichia coli | Porphyromonas gingivalis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
release of an N-terminal dipeptide, Xaa-Yaa-/-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline | conserved residues Ser624, Asp702, and His734 form a catalytic triad and are involved in the catalytic reaction, Ser593 is likely to be the catalytic serine of the serine protease | Porphyromonas gingivalis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.002 | - |
below, mutant enzymes | Porphyromonas gingivalis |
15 | - |
purified native enzyme | Porphyromonas gingivalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gly-Pro-4-nitroanilide + H2O | - |
Porphyromonas gingivalis | Gly-Pro + 4-nitroaniline | - |
? | |
additional information | the enzyme does not bind to adenosine deaminase, EC 3.5.4.4 | Porphyromonas gingivalis | ? | - |
? | |
QPDAINAP + H2O | peptide sequence corresponds to the N-terminus of human MCP1 protein | Porphyromonas gingivalis | Gln-Pro + DAINAP | - |
? | |
SPYSSDTT + H2O | peptide sequence corresponds to the N-terminus of human RANTES protein | Porphyromonas gingivalis | Ser-Pro + YSSDTT | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 74000, native enzyme, SDS-PAGE | Porphyromonas gingivalis |
Synonyms | Comment | Organism |
---|---|---|
dipeptidyl aminopeptidase IV | - |
Porphyromonas gingivalis |
DPPIV | - |
Porphyromonas gingivalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Porphyromonas gingivalis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
native enzyme, 30 min, 70% remaining activity | Porphyromonas gingivalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
native enzyme | Porphyromonas gingivalis |