Any feedback?
Literature summary for 3.4.14.5 extracted from
- Molecular characterization of dipeptidyl peptidase activity in serum. Soluble CD26/dipeptidyl peptidase IV is responsible for the release of X-Pro dipeptides (2000), Eur. J. Biochem., 267, 5608-5613.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in human K562 erythroleukemia cell line, secretion to the medium of the recombinant enzyme | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| di{4-[(erythrocarbonyl)methylaminoacrbonyl]-phenyl} 1-((S)-prolyl)pyrrolidine-2(R,S)-phosphonate | irreversible | Homo sapiens |  |
| N-isoleucyl-3-fluoro-pyrrolidine | reversible | Homo sapiens | |
| Zn2+ | competitive | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plasma membrane | - |
Homo sapiens | 5886 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 110000 | - |
x * 110000, enzyme from serum and seminal plasma, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P27487 | T-cell enzyme | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | heavily glycosylated, differing in the pattern dependent on the enzyme origin | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native from serum and seminal plasma, recombinant from K562 cell dulture medium | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| B-cell | - |
Homo sapiens | - |
| endothelium | - |
Homo sapiens | - |
| seminal plasma | - |
Homo sapiens | - |
| serum | - |
Homo sapiens | - |
| T-cell | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 41.9 | - |
purified native enzyme from serum | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Gly-Pro-4-methoxy-2-naphthylamide | fluorogenic substrate | Homo sapiens | Gly-Pro + 4-methoxy-2-naphthylamine | - |
? | |
| Gly-Pro-4-nitroanilide + H2O | - |
Homo sapiens | Gly-Pro + 4-nitroaniline | - |
? | |
| additional information | the enzyme binds with high affinity to adenosine deaminase, EC 3.5.4.4 | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 110000, enzyme from serum and seminal plasma, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CD26 | enzyme is identical with adenosine deaminase binding protein, CD26 | Homo sapiens |
| dipeptidyl peptidase IV | - |
Homo sapiens |
| DPPIV | - |
Homo sapiens |
| More | the enzyme is not identical with attractin | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
5% remaining activity after 100 min, slightly differing thermal stability for the 3 different purified enzyme forms from serum, seminal plasma and recombinant from K562 cells | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
about | Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | no activity below pH 5.0, optimum at pH 8.0 | Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
