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Literature summary for 3.4.14.5 extracted from

  • Khalid, N.M.; Marth, E.H.
    Purification and partial characterization of a prolyl-dipeptidyl aminopeptidase from Lactobacillus helveticus CNRZ 32 (1990), Appl. Environ. Microbiol., 56, 381-388.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.14.5

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 1.0 mM, 14 mM 40°C, partial Lactobacillus helveticus
Cu2+ 0.1-2.5 mM Lactobacillus helveticus
diisopropyl fluorophosphate 1.0 mM, 2 h, 40°C, complete inhibition Lactobacillus helveticus
EDTA 1.0 mM, 40°C, 20 h, partial Lactobacillus helveticus
Hg2+ 0.1-2.5 mM Lactobacillus helveticus
iodoacetate 1.0 mM, partial Lactobacillus helveticus
NEM 1.0 mM, partial Lactobacillus helveticus
PCMB 1.0 mM, 15 min, 40°C, complete inhibition Lactobacillus helveticus
phenylmethylsulfonyl fluoride 1.0 mM, partial Lactobacillus helveticus
Zn2+ 0.1-2.5 mM Lactobacillus helveticus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 0.1-2.5 mM, activates Lactobacillus helveticus
K+ 10-50 mM, activates Lactobacillus helveticus
Mg2+ 2.5 mM, activates Lactobacillus helveticus
Na+ 10-50 mM, activates Lactobacillus helveticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
 non-denaturing PAGE Lactobacillus helveticus

Organism

Organism UniProt Comment Textmining
Lactobacillus helveticus
-
 CNRZ 32
-
Lactobacillus helveticus CNRZ 32
-
 CNRZ 32
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lactobacillus helveticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Lactobacillus helveticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-4-nitroanilide + H2O 2% of the activity with Gly-Pro-4-nitroanilide Lactobacillus helveticus Ala-Ala + 4-nitroaniline
-
 ?
Ala-Ala-4-nitroanilide + H2O 2% of the activity with Gly-Pro-4-nitroanilide Lactobacillus helveticus CNRZ 32 Ala-Ala + 4-nitroaniline
-
 ?
Ala-Pro-Gly + H2O 11% of the activity with Gly-Pro-4-nitroanilide Lactobacillus helveticus Ala-Pro + Gly
-
 ?
Ala-Pro-Gly + H2O 11% of the activity with Gly-Pro-4-nitroanilide Lactobacillus helveticus CNRZ 32 Ala-Pro + Gly
-
 ?
Arg-Pro-4-nitroanilide + H2O 130% of the activity with Gly-Pro-4-nitroanilide Lactobacillus helveticus Arg-Pro + 4-nitroaniline
-
 ?
Arg-Pro-4-nitroanilide + H2O 130% of the activity with Gly-Pro-4-nitroanilide Lactobacillus helveticus CNRZ 32 Arg-Pro + 4-nitroaniline
-
 ?
Gly-Pro-4-nitroanilide + H2O
-
 Lactobacillus helveticus Gly-Pro + 4-nitroaniline
-
 ?
Gly-Pro-4-nitroanilide + H2O
-
 Lactobacillus helveticus CNRZ 32 Gly-Pro + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 72000, SDS-PAGE Lactobacillus helveticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Lactobacillus helveticus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 60 about 40% of maximal activity at 5°C and at 60°C Lactobacillus helveticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 hydrolysis of Gly-Pro-p-nitroanilide Lactobacillus helveticus

pH Range

pH Minimum pH Maximum Comment Organism
5 8.5 pH 5.0: about 50% of maximal activity, pH 8.5: about 60% of maximal activity Lactobacillus helveticus