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Literature summary for 3.4.14.12 extracted from

  • Umezawa, Y.; Yokoyama, K.; Kikuchi, Y.; Date, M.; Ito, K.; Yoshimoto, T.; Matsui, H.
    Novel prolyl tri/tetra-peptidyl aminopeptidase from Streptomyces mobaraensis: substrate specificity and enzyme gene cloning (2004), J. Biochem., 136, 293-300.
    View publication on PubMed
Search for more literature for 3.4.14.12

Cloned(Commentary)

Cloned (Comment) Organism
-
 Streptomyces mobaraensis

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 5 mM, 67% residual activity Streptomyces mobaraensis
Pefabloc SC 4 mM, 60% residual activity Streptomyces mobaraensis
Phenylmethylsulfonylfluoride 1 mM, no residual activity Streptomyces mobaraensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.072
-
 Gly-L-Ala-L-Pro-beta-naphthylamide pH 6.5, 30°C Streptomyces mobaraensis
0.26
-
 Gly-L-Ala-Gly-L-Pro-beta-naphthylamide pH 6.5, 30°C Streptomyces mobaraensis

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall connnected to wall through ionic interactions Streptomyces mobaraensis 5618
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 x * 50000, SDS-PAGE Streptomyces mobaraensis

Organism

Organism UniProt Comment Textmining
Streptomyces mobaraensis
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification sequence contains an N-terminal signal peptide of 33 amino acids followed by mature protein of 444 amino acids Streptomyces mobaraensis

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces mobaraensis

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Streptomyces mobaraensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly-L-Ala-Gly-L-Pro-beta-naphthylamide + H2O
-
 Streptomyces mobaraensis Gly-L-Ala-Gly-L-Pro + beta-naphthylamine
-
 ?
Gly-L-Ala-L-Pro-4-nitroanilide + H2O 32% of the activity with L-Ala-L-Ala-L-Pro-4-nitroanilide Streptomyces mobaraensis Gly-L-Ala-L-Pro + 4-nitroaniline
-
 ?
Gly-L-Ala-L-Pro-beta-naphthylamide + H2O
-
 Streptomyces mobaraensis Gly-L-Ala-L-Pro + beta-naphthylamine
-
 ?
L-Ala-L-Ala-L-Pro-4-nitroanilide + H2O
-
 Streptomyces mobaraensis L-Ala-L-Ala-L-Pro + 4-nitroaniline
-
 ?
L-Ala-L-Phe-L-Pro-4-nitroanilide + H2O 24% of the activity with L-Ala-L-Ala-L-Pro-4-nitroanilide Streptomyces mobaraensis L-Ala-L-Phe-L-Pro + 4-nitroaniline
-
 ?
L-Lys-L-Pro-L-Pro-4-nitroanilide + H2O 26% of the activity with L-Ala-L-Ala-L-Pro-4-nitroanilide Streptomyces mobaraensis L-Pro-L-Pro-L-Pro + 4-nitroaniline
-
 ?
additional information enzyme exhibits tripeptidyl peptidase and tetrapeptidyl peptidase activity, but no other exo- or endo-activites Streptomyces mobaraensis ?
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Streptomyces mobaraensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Streptomyces mobaraensis
50
-
 significant decrease in activity above Streptomyces mobaraensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 stable Streptomyces mobaraensis
40
-
 significant loss of stability above Streptomyces mobaraensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
43
-
 Gly-L-Ala-Gly-L-Pro-beta-naphthylamide pH 6.5, 30°C Streptomyces mobaraensis
115
-
 Gly-L-Ala-L-Pro-beta-naphthylamide pH 6.5, 30°C Streptomyces mobaraensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5
-
 Streptomyces mobaraensis

pH Range

pH Minimum pH Maximum Comment Organism
4.3 8.3
-
 Streptomyces mobaraensis

pI Value

Organism Comment pI Value Maximum pI Value
Streptomyces mobaraensis isoelectric focusing
-
 10.2