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Literature summary for 3.4.14.12 extracted from

  • Fujimura, S.; Ueda, O.; Shibata, Y.; Hirai, K.
    Isolation and properties of a tripeptidyl peptidase from a periodontal pathogen Prevotella nigrescens (2003), FEMS Microbiol. Lett., 219, 305-309.
    View publication on PubMed
Search for more literature for 3.4.14.12

Inhibitors

Inhibitors Comment Organism Structure
3,4-dichloroisocoumarin 5.0 mM, 36% inhibition Prevotella nigrescens
diisopropyl fluorophosphate 5.0 mM, 69% inhibition Prevotella nigrescens
additional information 1 mM NEM, 5 mM 1,10-phenanthroline, 1 mM EDTA, 0.2 mM E64, 0.4 mM antipain, 0.2 mM leupeptin, 1 mg/ml aprotinin, 0.2 mM bestatin or 1 mM mercaptoethanol; no inhibition by Prevotella nigrescens
Pefabloc SC 1.0 mM, 33% inhibition Prevotella nigrescens
tosyl-L-lysine chloromethyl ketone 1.0 mM, 47% inhibition Prevotella nigrescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.069
-
 Ala-Ala-Pro-p-nitroanilide pH 7.5, 37°C Prevotella nigrescens
0.084
-
 Lys-Pro-p-nitroanilide pH 7.5, 37°C Prevotella nigrescens
0.114
-
 Arg-Pro-p-nitroanilide pH 7.5, 37°C Prevotella nigrescens
0.129
-
 Ala-Phe-Pro-p-nitroanilide pH 7.5, 37°C Prevotella nigrescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 x * 56000, SDS-PAGE Prevotella nigrescens

Organism

Organism UniProt Comment Textmining
Prevotella nigrescens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Prevotella nigrescens

Storage Stability

Storage Stability Organism
-40°C, stable for several weeks Prevotella nigrescens
4°C, stable for 24 h Prevotella nigrescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-Pro-p-nitroanilide + H2O
-
 Prevotella nigrescens Ala-Ala-Pro + p-nitroaniline
-
 ?
Ala-Phe-Pro-p-nitroanilide + H2O 66% of the activity with Ala-Ala-Pro-p-nitroanilide Prevotella nigrescens Ala-Phe-Pro + p-nitroaniline
-
 ?
Ala-Pro-p-nitroanilide + H2O 13% of the activity with Ala-Ala-Pro-p-nitroanilide Prevotella nigrescens Ala-Pro + p-nitroaniline
-
 ?
Arg-Pro-p-nitroanilide + H2O 22% of the activity with Ala-Ala-Pro-p-nitroanilide Prevotella nigrescens Arg-Pro + p-nitroaniline
-
 ?
Gly-Pro-p-nitroanilide + H2O 3% of the activity with Ala-Ala-Pro-p-nitroanilide Prevotella nigrescens Gly-Pro + p-nitroaniline
-
 ?
Lys-Pro-p-nitroanilide + H2O 24% of the activity with Ala-Ala-Pro-p-nitroanilide Prevotella nigrescens Lys-Pro + p-nitroaniline
-
 ?
additional information no activity with Ala-Phe-p-nitroanilide, Ala-Ala-p-nitroanilide, Ala-p-nitroanilide, Pro-p-nitroanilide, benzoyl-Arg-p-nitroanilide, succinyl-Ala-Ala-Ala-p-nitroanilide, succinyl-Ala-Ala-Pro-p-nitroanilide, tosyl-Gly-Pro-Lys-4-nitroanilide Prevotella nigrescens ?
-
 ?
Phe-Pro-p-nitroanilide + H2O 13% of the activity with Ala-Ala-Pro-p-nitroanilide Prevotella nigrescens Phe-Pro + p-nitroaniline
-
 ?
Ser-Pro-p-nitroanilide + H2O 13% of the activity with Ala-Ala-Pro-p-nitroanilide Prevotella nigrescens Ser-Pro + p-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 56000, SDS-PAGE Prevotella nigrescens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 5 min, complete loss of activity Prevotella nigrescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
 Prevotella nigrescens

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5 no significant activity is observed below pH 6.0 and above pH 8.5 Prevotella nigrescens