Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,4-dichloroisocoumarin | 1.0 mM, complete inhibition | Latilactobacillus sakei | |
Cu2+ | 1 mM CuCl2, 69% inhibition | Latilactobacillus sakei | |
Hg2+ | 1 mM HgCl2, 78% inhibition | Latilactobacillus sakei | |
phenylmethylsulfonyl fluoride | 1 mM, 43% inhibition | Latilactobacillus sakei | |
Zn2+ | 0.1 mM, 50% inhibition | Latilactobacillus sakei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
Gly-Pro-7-amido-4-methylcoumarin | pH 7.5, 37°C | Latilactobacillus sakei | |
0.05 | - |
Ala-Pro-4-nitroanilide | pH 7.5, 37°C | Latilactobacillus sakei | |
0.088 | - |
Lys-Ala-7-amido-4-methylcoumarin | pH 7.5, 37°C | Latilactobacillus sakei | |
0.192 | - |
Gly-Pro-4-nitroanilide | pH 7.5, 37°C | Latilactobacillus sakei |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not a metalloenzyme | Latilactobacillus sakei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
88000 | - |
2 * 88000, SDS-PAGE | Latilactobacillus sakei |
170000 | - |
gel filtration | Latilactobacillus sakei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Latilactobacillus sakei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Latilactobacillus sakei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
236.5 | - |
- |
Latilactobacillus sakei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Arg-Pro 4-nitroanilide + H2O | 60.5% of the activity with Gly-Pro-7-amido-4-methylcoumarin | Latilactobacillus sakei | Arg-Pro + 4-nitroaniline | - |
? | |
Gly-Pro 4-nitroanilide + H2O | 25.5% of the activity with Gly-Pro-7-amido-4-methylcoumarin | Latilactobacillus sakei | Gly-Pro + 4-nitroaniline | - |
? | |
Gly-Pro-7-amido-4-methylcoumarin + H2O | - |
Latilactobacillus sakei | Gly-Pro + 7-amino-4-methylcoumarin | - |
? | |
Gly-Pro-Ala + H2O | - |
Latilactobacillus sakei | Gly-Pro + Ala | - |
? | |
L-Ala-L-Pro-4-nitroanilide + H2O | best substrate | Latilactobacillus sakei | L-Ala-L-Pro + 4-nitroaniline | - |
? | |
Lys-Ala-7-amido-4-methylcoumarin + H2O | 9.9% of the activity with Gly-Pro-7-amido-4-methylcoumarin | Latilactobacillus sakei | Lys-Ala + 7-amino-4-methylcoumarin | - |
? | |
additional information | maximal activity with Pro in the N-penultimate position | Latilactobacillus sakei | ? | - |
? | |
Tyr-Pro-Phe + H2O | - |
Latilactobacillus sakei | Tyr-Pro + Phe | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 88000, SDS-PAGE | Latilactobacillus sakei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Latilactobacillus sakei |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 65 | about 30% of maximal activity at 20°C and at 65°C, hydrolysis of Gly-Pro-7-amido-4-methylcoumarin | Latilactobacillus sakei |
35 | 60 | 35°C: about 50% of maximal activity, 60°C: about 30% of maximal activity, hydrolysis of Gly-Pro-7-amido-4-methylcoumarin | Latilactobacillus sakei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Latilactobacillus sakei |