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Literature summary for 3.4.14.11 extracted from

  • Tachi, H.; Ito, H.; Ichishima, E.
    An X-prolyl dipeptidyl-aminopeptidase from Aspergillus oryzae (1992), Phytochemistry, 31, 3707-3709.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
diisopropylfluorophosphate
-
Aspergillus oryzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.55
-
Gly-Pro 4-nitroanilide
-
Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
145000
-
2 * 145000, SDS-PAGE Aspergillus oryzae
280000
-
gel filtration Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus oryzae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala 4-nitroanilide + H2O
-
Aspergillus oryzae Ala-Ala + 4-nitroaniline
-
?
Arg-Pro 4-nitroanilide + H2O best substrate Aspergillus oryzae Arg-Pro + 4-nitroaniline
-
?
Gly-Pro 4-nitroanilide + H2O
-
Aspergillus oryzae Gly-Pro + 4-nitroaniline
-
?

Subunits

Subunits Comment Organism
dimer 2 * 145000, SDS-PAGE Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5 hydrolysis of Gly-Pro 4-nitroanilide Aspergillus oryzae

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 9 10 min, stable Aspergillus oryzae

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus oryzae
-
-
4.2