Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DMSO | up to 25%, enhances activity | Lactococcus lactis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diisopropylfluorophosphate | - |
Lactococcus lactis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Lactococcus lactis | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85000 | - |
2 * 85000, SDS-PAGE with or without 2-mercaptoethanol | Lactococcus lactis |
190000 | - |
gel filtration | Lactococcus lactis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | - |
ssp. lactis | - |
Lactococcus lactis | - |
NCDO 763 | - |
Lactococcus lactis NCDO 763 | - |
NCDO 763 | - |
Purification (Comment) | Organism |
---|---|
- |
Lactococcus lactis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Lactococcus lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Arg-Pro 4-nitroanilide + H2O | - |
Lactococcus lactis | Arg-Pro + 4-nitroaniline | - |
? | |
beta-casomorphin + H2O | Tyr-Pro-Phe-Pro-Gly-Pro-Ile | Lactococcus lactis | ? | the hydrolysis of the tripeptide Gly-Pro-Ile is slower | ? | |
beta-casomorphin + H2O | Tyr-Pro-Phe-Pro-Gly-Pro-Ile | Lactococcus lactis | ? | after the release of the dipeptide Tyr-Pro, the resulting pentapeptide does not accumulate in the medium, rather the dipeptide Phe-Pro is released very rapidly | ? | |
beta-casomorphin + H2O | Tyr-Pro-Phe-Pro-Gly-Pro-Ile | Lactococcus lactis NCDO 763 | ? | the hydrolysis of the tripeptide Gly-Pro-Ile is slower | ? | |
Gly-Pro 4-nitroanilide + H2O | - |
Lactococcus lactis | Gly-Pro + 4-nitroaniline | - |
? | |
L-Ala-L-Pro-4-nitroanilide + H2O | - |
Lactococcus lactis | L-Ala-L-Pro + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 85000, SDS-PAGE with or without 2-mercaptoethanol | Lactococcus lactis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Lactococcus lactis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | 50% of maximal activity at 30°C, maximum at 40-50°C | Lactococcus lactis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Lactococcus lactis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | pH 5.5: 50% of maximal activity, pH 8.5: activity maximum | Lactococcus lactis |