Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.14.11 extracted from

  • Kiefer-Partsch, B.; Bockelmann, W.; Geis, A.; Teuber, M.
    Purification of an X-prolyl-dipeptidyl aminopeptidase from the cell wall proteolytic system of Lactococcus lactis subsp. Cremoris (1989), Appl. Microbiol. Biotechnol., 31, 75-78.
No PubMed abstract available
Search for more literature for 3.4.14.11

Inhibitors

Inhibitors Comment Organism Structure
diisopropylfluorophosphate
-
 Lactococcus lactis
EDTA no inhibition Lactococcus lactis
phenylmethylsulfonyl fluoride
-
 Lactococcus lactis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information metal-independent enzyme Lactococcus lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 2 * 90000, SDS-PAGE Lactococcus lactis
160000 180000 gel filtration Lactococcus lactis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-casein + H2O Lactococcus lactis function of the enzyme is to cleave the proline-rich sequence of beta-casein ?
-
 ?

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
 subsp. cremoris
-
Lactococcus lactis
-
 P8-2-47
-
Lactococcus lactis P8-2-47
-
 P8-2-47
-
Lactococcus lactis subsp. cremoris
-
 subsp. cremoris
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lactococcus lactis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
90
-
-
 Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-casein + H2O function of the enzyme is to cleave the proline-rich sequence of beta-casein Lactococcus lactis ?
-
 ?
beta-casomorphin + H2O
-
 Lactococcus lactis ? main split products: Tyr-Pro and Phe-Pro ?
beta-casomorphin + H2O
-
 Lactococcus lactis P8-2-47 ? main split products: Tyr-Pro and Phe-Pro ?
beta-casomorphin + H2O
-
 Lactococcus lactis subsp. cremoris ? main split products: Tyr-Pro and Phe-Pro ?
L-Ala-L-Pro-4-nitroanilide + H2O
-
 Lactococcus lactis L-Ala-L-Pro + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 90000, SDS-PAGE Lactococcus lactis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Lactococcus lactis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 8
-
 Lactococcus lactis