Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the subunits form an active complexwith a MW of 1000000 Da, the complex can sponateously dissociate in vitro into dimers which retain 1/10th of the original specific activity. The dissociated enzyme can reassociate at elevated temperatures, provided the protein concentration is sufficiently high. This reassiciation is accompanied by a reactivation. At 30°C more than 7fold activation after 2 h. Arg-Arg-Ala and Ser-Val-Ala at 0.01 mM enhance reactivation | Homo sapiens |
General Stability | Organism |
---|---|
purified enzyme undergos a spontaneous dissociation upon storage, the dissociated enzyme has a specific activity which is 1/10th of that of the normal high MW complex | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Arg-Ala-DELTAAla-Val-Ala | competitive | Homo sapiens | |
Arg-Arg-Ala | - |
Homo sapiens | |
butabindide | competitive | Homo sapiens | |
Ser-Val-Ala | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
Ala-Ala-Phe-p-nitroanilide | pH 7.5, 37°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
138000 | - |
x * 138000, the subunits form an active complex with a MW of 1000000 Da, the complex can sponateously dissociate in vitro into dimers which retain 1/10th of the original specific activity. The dissociated enzyme can reassociate at elevated temperatures, provided the protein concentration is sufficiently high. This reassociation is accompanied by a reactivation | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | association/dissociation may be a way of regulating the enzyme activity in vivo | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Homo sapiens | - |
Storage Stability | Organism |
---|---|
-70°C, stable for 4 years | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Ala-Phe-p-nitroanilide + H2O | - |
Homo sapiens | Ala-Ala-Phe + p-nitroaniline | - |
? | |
additional information | association/dissociation may be a way of regulating the enzyme activity in vivo | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 138000, the subunits form an active complex with a MW of 1000000 Da, the complex can sponateously dissociate in vitro into dimers which retain 1/10th of the original specific activity. The dissociated enzyme can reassociate at elevated temperatures, provided the protein concentration is sufficiently high. This reassociation is accompanied by a reactivation | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
Arg-Arg-Ala | pH 7.5, 37°C | Homo sapiens | |
0.01 | - |
Ser-Val-Ala | pH 7.5, 37°C | Homo sapiens |