Crystallization (Comment) | Organism |
---|---|
structures from crystals with no additional metal added, Mn2+ soaked crystals and Zn2+ soaked crystals solved to 1.83 A, 1.85 A, and 1.95 A resolution, respectively | Xanthomonas campestris pv. campestris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
His-Pro | substrate inhibition | Xanthomonas campestris pv. campestris | |
Leu-Pro | substrate inhibition | Xanthomonas campestris pv. campestris | |
Lys-Pro | substrate inhibition | Xanthomonas campestris pv. campestris | |
Pro-Pro | substrate inhibition | Xanthomonas campestris pv. campestris | |
Tyr-Pro | substrate inhibition | Xanthomonas campestris pv. campestris | |
Zn2+ | inhibitory above 0.01 mM | Xanthomonas campestris pv. campestris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
Pro-Pro | pH 7.5, 55°C | Xanthomonas campestris pv. campestris | |
0.9 | - |
His-Pro | pH 7.5, 55°C | Xanthomonas campestris pv. campestris | |
1.2 | - |
Leu-Pro | pH 7.5, 55°C | Xanthomonas campestris pv. campestris | |
1.9 | - |
Val-Pro | pH 7.5, 55°C | Xanthomonas campestris pv. campestris | |
2.1 | - |
Lys-Pro | pH 7.5, 55°C | Xanthomonas campestris pv. campestris | |
3.1 | - |
Met-Pro | pH 7.5, 55°C | Xanthomonas campestris pv. campestris | |
3.3 | - |
Phe-Pro | pH 7.5, 55°C | Xanthomonas campestris pv. campestris | |
3.4 | - |
Gly-Pro | pH 7.5, 55°C | Xanthomonas campestris pv. campestris | |
5.9 | - |
Arg-Pro | pH 7.5, 55°C | Xanthomonas campestris pv. campestris | |
6.6 | - |
Leu-Pro | pH 7.5, 55°C | Mycobacterium tuberculosis | |
7 | - |
Tyr-Pro | pH 7.5, 55°C | Xanthomonas campestris pv. campestris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | maximum activity in presence of Mn2+ ions, addition of 1 mM to the assay | Xanthomonas campestris pv. campestris | |
Mn2+ | maximum activity in presence of Mn2+ ions, addition of 1 mM to the assay | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WHS7 | - |
- |
Mycobacterium tuberculosis ATCC 25618 | P9WHS7 | - |
- |
Xanthomonas campestris pv. campestris | Q8P839 | - |
- |
Xanthomonas campestris pv. campestris DSM 3586 | Q8P839 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Arg-Pro + H2O | - |
Xanthomonas campestris pv. campestris | Arg + Pro | - |
? | |
Arg-Pro + H2O | - |
Xanthomonas campestris pv. campestris DSM 3586 | Arg + Pro | - |
? | |
Gly-Pro + H2O | - |
Xanthomonas campestris pv. campestris | Gly + Pro | - |
? | |
His-Pro + H2O | - |
Xanthomonas campestris pv. campestris | His + Pro | - |
? | |
Leu-Pro + H2O | - |
Xanthomonas campestris pv. campestris | Leu + Pro | - |
? | |
Leu-Pro + H2O | best substrate | Mycobacterium tuberculosis | Leu + Pro | - |
? | |
Leu-Pro + H2O | best substrate | Mycobacterium tuberculosis ATCC 25618 | Leu + Pro | - |
? | |
Leu-Pro + H2O | - |
Xanthomonas campestris pv. campestris DSM 3586 | Leu + Pro | - |
? | |
Lys-Pro + H2O | - |
Xanthomonas campestris pv. campestris | Lys + Pro | - |
? | |
Met-Pro + H2O | - |
Xanthomonas campestris pv. campestris | Met + Pro | - |
? | |
Met-Pro + H2O | - |
Mycobacterium tuberculosis | Met + Pro | - |
? | |
Met-Pro + H2O | - |
Mycobacterium tuberculosis ATCC 25618 | Met + Pro | - |
? | |
Met-Pro + H2O | - |
Xanthomonas campestris pv. campestris DSM 3586 | Met + Pro | - |
? | |
additional information | decreasing order of preference for the N-terminal residue of the Xaa-Pro dipeptides in presence of Mn2+ ions is Leu, Pro, Phe, Met, Arg, Lys, Ile, Ser, His, Ala, Gly, Val, Asp while in that of Zn2+ ions is Leu, Phe, Met, Pro, Arg, Lys, Ala, Ile, Ser, Gly, Val, Asp | Mycobacterium tuberculosis | ? | - |
? | |
additional information | enzyme exhibits allosteric behavior and substrate inhibition toward dipeptides Leu-Pro, Pro-Pro, His-Pro, Lys-Pro and Tyr-Pro. The order of preference for the N-terminal residue of the Xaa-Pro dipeptides is similar in presence of Mn2+ ions (in decreasing order: Phe, Ser, Met, Leu, Ala, Pro, His, Arg, Ile, Gly, Val, Asp) and Zn2+ ions (0.005 mM) (Met, Phe, SerN, Leu, Ala, Pro, Arg, Lys, Ile=Gly, Val, Asp). The activity towards all the substrates is 4-5 times higher in presence of Mn2+ than Zn2+ ions | Xanthomonas campestris pv. campestris | ? | - |
? | |
additional information | decreasing order of preference for the N-terminal residue of the Xaa-Pro dipeptides in presence of Mn2+ ions is Leu, Pro, Phe, Met, Arg, Lys, Ile, Ser, His, Ala, Gly, Val, Asp while in that of Zn2+ ions is Leu, Phe, Met, Pro, Arg, Lys, Ala, Ile, Ser, Gly, Val, Asp | Mycobacterium tuberculosis ATCC 25618 | ? | - |
? | |
additional information | enzyme exhibits allosteric behavior and substrate inhibition toward dipeptides Leu-Pro, Pro-Pro, His-Pro, Lys-Pro and Tyr-Pro. The order of preference for the N-terminal residue of the Xaa-Pro dipeptides is similar in presence of Mn2+ ions (in decreasing order: Phe, Ser, Met, Leu, Ala, Pro, His, Arg, Ile, Gly, Val, Asp) and Zn2+ ions (0.005 mM) (Met, Phe, SerN, Leu, Ala, Pro, Arg, Lys, Ile=Gly, Val, Asp). The activity towards all the substrates is 4-5 times higher in presence of Mn2+ than Zn2+ ions | Xanthomonas campestris pv. campestris DSM 3586 | ? | - |
? | |
Phe-Pro + H2O | - |
Xanthomonas campestris pv. campestris | Phe + Pro | - |
? | |
Phe-Pro + H2O | - |
Mycobacterium tuberculosis | Phe + Pro | - |
? | |
Phe-Pro + H2O | - |
Mycobacterium tuberculosis ATCC 25618 | Phe + Pro | - |
? | |
Phe-Pro + H2O | - |
Xanthomonas campestris pv. campestris DSM 3586 | Phe + Pro | - |
? | |
Pro-Pro + H2O | - |
Xanthomonas campestris pv. campestris | Pro + Pro | - |
? | |
Ser-Pro + H2O | - |
Xanthomonas campestris pv. campestris | Ser + Pro | - |
? | |
Ser-Pro + H2O | - |
Mycobacterium tuberculosis | Ser + Pro | - |
? | |
Ser-Pro + H2O | - |
Mycobacterium tuberculosis ATCC 25618 | Ser + Pro | - |
? | |
Tyr-Pro + H2O | - |
Xanthomonas campestris pv. campestris | Tyr + Pro | - |
? | |
Tyr-Pro + H2O | - |
Mycobacterium tuberculosis | Tyr + Pro | - |
? | |
Val-Pro + H2O | - |
Xanthomonas campestris pv. campestris | Val + Pro | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PepE | - |
Mycobacterium tuberculosis |
PepQ | - |
Xanthomonas campestris pv. campestris |
prolidase | - |
Xanthomonas campestris pv. campestris |
XPD | - |
Xanthomonas campestris pv. campestris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Mycobacterium tuberculosis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
100% loss of activity at 50°C in absence of Mn2+ | Mycobacterium tuberculosis |
50 | - |
35% loss of activity at 50°C in absence of Mn2+ | Xanthomonas campestris pv. campestris |
65 | - |
100% activity up to 65°C in presence of Mn2+ | Xanthomonas campestris pv. campestris |
65 | - |
50% loss of activity at 65°C in presence of Mn2+ | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1000 | - |
Leu-Pro | pH 7.5, 55°C | Mycobacterium tuberculosis | |
3000 | - |
Val-Pro | pH 7.5, 55°C | Xanthomonas campestris pv. campestris | |
3000 | - |
His-Pro | pH 7.5, 55°C | Xanthomonas campestris pv. campestris | |
4500 | - |
Pro-Pro | pH 7.5, 55°C | Xanthomonas campestris pv. campestris | |
5000 | - |
Leu-Pro | pH 7.5, 55°C | Xanthomonas campestris pv. campestris | |
5700 | - |
Lys-Pro | pH 7.5, 55°C | Xanthomonas campestris pv. campestris | |
6100 | - |
Gly-Pro | pH 7.5, 55°C | Xanthomonas campestris pv. campestris | |
9100 | - |
Met-Pro | pH 7.5, 55°C | Xanthomonas campestris pv. campestris | |
12500 | - |
Tyr-Pro | pH 7.5, 55°C | Xanthomonas campestris pv. campestris | |
16900 | - |
Phe-Pro | pH 7.5, 55°C | Xanthomonas campestris pv. campestris | |
19800 | - |
Arg-Pro | pH 7.5, 55°C | Xanthomonas campestris pv. campestris |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
200 | - |
Leu-Pro | pH 7.5, 55°C | Mycobacterium tuberculosis | |
1600 | - |
Val-Pro | pH 7.5, 55°C | Xanthomonas campestris pv. campestris | |
1800 | - |
Gly-Pro | pH 7.5, 55°C | Xanthomonas campestris pv. campestris | |
1800 | - |
Tyr-Pro | pH 7.5, 55°C | Xanthomonas campestris pv. campestris | |
2700 | - |
Lys-Pro | pH 7.5, 55°C | Xanthomonas campestris pv. campestris | |
2900 | - |
Met-Pro | pH 7.5, 55°C | Xanthomonas campestris pv. campestris | |
3300 | - |
Arg-Pro | pH 7.5, 55°C | Xanthomonas campestris pv. campestris | |
3300 | - |
His-Pro | pH 7.5, 55°C | Xanthomonas campestris pv. campestris | |
4200 | - |
Leu-Pro | pH 7.5, 55°C | Xanthomonas campestris pv. campestris | |
5100 | - |
Phe-Pro | pH 7.5, 55°C | Xanthomonas campestris pv. campestris | |
11200 | - |
Pro-Pro | pH 7.5, 55°C | Xanthomonas campestris pv. campestris |