Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus horikoshii |
Protein Variants | Comment | Organism |
---|---|---|
A195T/G306S | mutation causes an increase in Tm-value of 0.1°C. Mutation causes an 1.7fold increase of the catalytic efficiency towards Leu-Pro | Pyrococcus horikoshii |
E127G/E252D | mutation causes an decrease in Tm-value of 2.1°C. Mutation causes an 1.3fold increase of the catalytic efficiency towards Leu-Pro | Pyrococcus horikoshii |
E36V | mutation causes an increase in Tm-value of 0.6°C. Mutation causes an 1.1fold increase of the catalytic efficiency towards Leu-Pro | Pyrococcus horikoshii |
additional information | randomly mutated enzymes are prepared Tt obtain a better enzyme for organophosphorus nerve agent decontamination and to investigate the structural factors that may influence protein thermostability and thermoactivity | Pyrococcus horikoshii |
Y301C/K342N | mutation causes an decrease in Tm-value of 0.5°C. Mutation causes an 1.2fold decrease of the catalytic efficiency towards Leu-Pro | Pyrococcus horikoshii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.81 | - |
Leu-Pro | pH 7.0, 70°C, mutant enzyme A195T/G306S | Pyrococcus horikoshii | |
0.92 | - |
Leu-Pro | pH 7.0, 70°C, wild-type enzyme | Pyrococcus horikoshii | |
0.98 | - |
Leu-Pro | pH 7.0, 70°C, mutant enzyme E127G/E252D | Pyrococcus horikoshii | |
1.6 | - |
Leu-Pro | pH 7.0, 70°C, mutant enzyme E36V | Pyrococcus horikoshii | |
2.92 | - |
Leu-Pro | pH 7.0, 70°C, mutant enzyme Y301C/K342N | Pyrococcus horikoshii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | O58691 | - |
- |
Pyrococcus horikoshii DSM 12428 | O58691 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type enzyme and mutant enzymes A195T/G306S, Y301C/K342N, E127G/E252D and E36V | Pyrococcus horikoshii |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
809 | - |
substratwe: Leu-Pro, pH 7.0, 70°C, wild-type enzyme | Pyrococcus horikoshii |
1119 | - |
substrate: Leu-Pro, pH 7.0, 70°C, mutant enzyme E127G/E252D | Pyrococcus horikoshii |
1245 | - |
substrate: Leu-Pro, pH 7.0, 70°C, mutant enzyme A195T/G306S | Pyrococcus horikoshii |
1597 | - |
substrate: Leu-Pro, pH 7.0, 70°C, mutant enzyme E36V | Pyrococcus horikoshii |
2146 | - |
substrate: Leu-Pro, pH 7.0, 70°C, mutant enzyme Y301C/K342N | Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Pro + H2O | - |
Pyrococcus horikoshii | Ala + Pro | - |
? | |
Gly-Pro + H2O | - |
Pyrococcus horikoshii | Gly + Pro | - |
? | |
Gly-Pro + H2O | - |
Pyrococcus horikoshii DSM 12428 | Gly + Pro | - |
? | |
Leu-Pro + H2O | - |
Pyrococcus horikoshii | Leu + Pro | - |
? | |
Leu-Pro + H2O | - |
Pyrococcus horikoshii DSM 12428 | Leu + Pro | - |
? | |
Met-Pro + H2O | - |
Pyrococcus horikoshii | Met + Pro | - |
? | |
Met-Pro + H2O | - |
Pyrococcus horikoshii DSM 12428 | Met + Pro | - |
? | |
Phe-Pro + H2O | - |
Pyrococcus horikoshii | Phe + Pro | - |
? | |
Phe-Pro + H2O | - |
Pyrococcus horikoshii DSM 12428 | Phe + Pro | - |
? | |
Val-Pro + H2O | - |
Pyrococcus horikoshii | Val + Pro | - |
? | |
Val-Pro + H2O | - |
Pyrococcus horikoshii DSM 12428 | Val + Pro | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PH0974 | locus name | Pyrococcus horikoshii |
Ph1prol | - |
Pyrococcus horikoshii |
prolidase | - |
Pyrococcus horikoshii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
wild-type enzyme and mutant enzymes A195T/G306S, Y301C/K342N, E127G/E252D and E36V | Pyrococcus horikoshii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
112 | - |
Tm-value for mutant enzyme E127G/E252D is 112.2°C | Pyrococcus horikoshii |
114 | - |
Tm-value for wild-type enzyme is 114.3°C, Tm-value for mutant enzyme A195T/G306S is 114.4°C, Tm-value for mutant enzyme Y301C/K342N is 113.8°C | Pyrococcus horikoshii |
115 | - |
Tm-value for mutant enzyme E36V is 112.2°C | Pyrococcus horikoshii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1079 | - |
Leu-Pro | pH 7.0, 70°C, wild-type enzyme | Pyrococcus horikoshii | |
1492 | - |
Leu-Pro | pH 7.0, 70°C, mutant enzyme E127G/E252D | Pyrococcus horikoshii | |
1660 | - |
Leu-Pro | pH 7.0, 70°C, mutant enzyme A195T/G306S | Pyrococcus horikoshii | |
2129 | - |
Leu-Pro | pH 7.0, 70°C, mutant enzyme E36V | Pyrococcus horikoshii | |
2861 | - |
Leu-Pro | pH 7.0, 70°C, mutant enzyme Y301C/K342N | Pyrococcus horikoshii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Pyrococcus horikoshii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
980 | - |
Leu-Pro | pH 7.0, 70°C, mutant enzyme Y301C/K342N | Pyrococcus horikoshii | |
1172 | - |
Leu-Pro | pH 7.0, 70°C, wild-type enzyme | Pyrococcus horikoshii | |
1331 | - |
Leu-Pro | pH 7.0, 70°C, mutant enzyme E36V | Pyrococcus horikoshii | |
1522 | - |
Leu-Pro | pH 7.0, 70°C, mutant enzyme E127G/E252D | Pyrococcus horikoshii | |
2049 | - |
Leu-Pro | pH 7.0, 70°C, mutant enzyme A195T/G306S | Pyrococcus horikoshii |