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Literature summary for 3.4.13.9 extracted from
- Characterization of two proline dipeptidases (prolidases) from the hyperthermophilic archaeon Pyrococcus horikoshii (2010), Appl. Microbiol. Biotechnol., 86, 177-188.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene PH0974, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21 (lambdaDE3) | Pyrococcus horikoshii |
| gene PH1149, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21 (lambdaDE3) | Pyrococcus horikoshii |
| gene PH1902, DNA and amino acid sequence determination and analysis | Pyrococcus horikoshii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.9 | - |
Met-Pro | pH 7.0, 100°C | Pyrococcus horikoshii |  |
| 3.4 | - |
Met-Pro | pH 7.0, 100°C | Pyrococcus horikoshii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Pyrococcus horikoshii | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | dependent on | Pyrococcus horikoshii | |
| Cu2+ | activates | Pyrococcus horikoshii | |
| Fe2+ | activates | Pyrococcus horikoshii | |
| Mn2+ | activates | Pyrococcus horikoshii | |
| Ni2+ | activates | Pyrococcus horikoshii | |
| Zn2+ | activates | Pyrococcus horikoshii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
x * 40000, recombinant enzyme, SDS-PAGE | Pyrococcus horikoshii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus horikoshii | O58885 | PH1149 or PepQ; isozyme PH1149 | - |
| Pyrococcus horikoshii | O59565 | PH1902; isozyme PH1902 | - |
| Pyrococcus horikoshii | P81535 | PH0974 or PepQ; gene pepQ, isozyme PH0974 or PepQ | - |
| Pyrococcus horikoshii ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3 / 700860D-5 | O58885 | PH1149 or PepQ; isozyme PH1149 | - |
| Pyrococcus horikoshii ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3 / 700860D-5 | P81535 | PH0974 or PepQ; gene pepQ, isozyme PH0974 or PepQ | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
substrate and metal specificities, recombinant enzyme, overview | Pyrococcus horikoshii |
| 1938 | - |
purified recombinant enzyme, 100°C, substrate Met-Pro | Pyrococcus horikoshii |
| 2355 | - |
purified recombinant enzyme, 100°C, substrate Met-Pro | Pyrococcus horikoshii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Ala-Pro + H2O | - |
Pyrococcus horikoshii | Ala + Pro | - |
? | |
| Arg-Pro + H2O | - |
Pyrococcus horikoshii | Arg + Pro | - |
? | |
| Arg-Pro + H2O | - |
Pyrococcus horikoshii ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3 / 700860D-5 | Arg + Pro | - |
? | |
| Gly-Pro + H2O | - |
Pyrococcus horikoshii | Gly + Pro | - |
? | |
| Leu-Pro + H2O | high activity | Pyrococcus horikoshii | Leu + Pro | - |
? | |
| Leu-Pro + H2O | high activity | Pyrococcus horikoshii ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3 / 700860D-5 | Leu + Pro | - |
? | |
| Met-Pro + H2O | best substrate | Pyrococcus horikoshii | Met + Pro | - |
? | |
| Met-Pro + H2O | best substrate | Pyrococcus horikoshii ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3 / 700860D-5 | Met + Pro | - |
? | |
| additional information | substrate specificity, overview | Pyrococcus horikoshii | ? | - |
? | |
| additional information | substrate specificity, overview | Pyrococcus horikoshii ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3 / 700860D-5 | ? | - |
? | |
| Phe-Pro + H2O | - |
Pyrococcus horikoshii | Phe + Pro | - |
? | |
| Pro-Ala + H2O | very low activity | Pyrococcus horikoshii | Pro + Ala | - |
? | |
| Pro-Ala + H2O | very low activity | Pyrococcus horikoshii ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3 / 700860D-5 | Pro + Ala | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, recombinant enzyme, SDS-PAGE | Pyrococcus horikoshii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PepQ | - |
Pyrococcus horikoshii |
| PH0974 | - |
Pyrococcus horikoshii |
| PH1149 | - |
Pyrococcus horikoshii |
| Ph1prol | - |
Pyrococcus horikoshii |
| Phprol | - |
Pyrococcus horikoshii |
| prolidase | - |
Pyrococcus horikoshii |
| prolidase homolog 1 | - |
Pyrococcus horikoshii |
| prolidase homolog 2 | - |
Pyrococcus horikoshii |
| Proline dipeptidase | - |
Pyrococcus horikoshii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | 100 | assay at | Pyrococcus horikoshii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
purified recombinant enzyme, t1/2 is 21 h | Pyrococcus horikoshii |
| 90 | - |
purified recombinant enzyme, t1/2 is 21.5 h | Pyrococcus horikoshii |
| 100 | - |
purified recombinant enzyme, 8 h, 100% stable | Pyrococcus horikoshii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2617 | - |
Met-Pro | pH 7.0, 100°C | Pyrococcus horikoshii | |
| 3812 | - |
Met-Pro | pH 7.0, 100°C | Pyrococcus horikoshii | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
two peaks at pH 5.0 and pH 7.0 | Pyrococcus horikoshii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | activity profile, overview | Pyrococcus horikoshii |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 768 | - |
Met-Pro | pH 7.0, 100°C | Pyrococcus horikoshii | |
| 2006 | - |
Met-Pro | pH 7.0, 100°C | Pyrococcus horikoshii | |
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Release 2023.1 (January 2023)
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