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Literature summary for 3.4.13.9 extracted from

  • Bielawski, K.; Bielawska, A.; S?odownik, T.; Bo?kun-Skornicka, U.; Muszy?ska, A.
    Proline-linked nitrosoureas as prolidase-convertible prodrugs in human breast cancer cells (2008), Pharmacol. Rep., 60, 171-182.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
N-[N'-(2-bromoethyl)-N'-nitrosocarbamoyl]-L-proline significantly increases MCF-7 cells prolidase activity, when used at 0.05-0.25 mM concentrations Homo sapiens
N-[N'-(2-chloroethyl)-N'-nitrosocarbamoyl]-L-proline significantly increases MCF-7 cells prolidase activity, when used at 0.05-0.25 mM concentrations Homo sapiens
N-[N'-(3-chloropropyl)-N'-nitrosocarbamoyl]-L-proline significantly increases MCF-7 cells prolidase activity, when used at 0.05-0.25 mM concentrations Homo sapiens
N-[N'-(4-bromophenyl)-N'-nitrosocarbamoyl]-L-proline significantly increases MCF-7 cells prolidase activity, when used at 0.05-0.25 mM concentrations Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
carmustine
-
Sus scrofa
additional information carmustine does not inhibit MCF-7 cells prolidase activity Homo sapiens
N-[N'-(2-bromoethyl)-N'-nitrosocarbamoyl]-L-proline
-
Sus scrofa
Z-Pro roughly 80% inhibition at 2.53 mM Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-
cytosol
-
Sus scrofa 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activation of prolidase requires preincubation with 2 mM Mn2+ Homo sapiens
Mn2+ activation of prolidase requires preincubation with 2 mM Mn2+ Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
SDS-PAGE Homo sapiens
56000
-
SDS-PAGE Sus scrofa

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
breast cancer cell
-
Homo sapiens
-
kidney
-
Sus scrofa
-
MCF-7 cell
-
Homo sapiens
-
MDA-MB-231 cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly-L-Pro + H2O
-
Homo sapiens Gly + L-Pro
-
?
Gly-L-Pro + H2O
-
Sus scrofa Gly + L-Pro
-
?
glycyl-L-hydroxyproline + H2O
-
Homo sapiens Gly + L-hydroxyproline
-
?
glycyl-L-hydroxyproline + H2O
-
Sus scrofa Gly + L-hydroxyproline
-
?

Synonyms

Synonyms Comment Organism
prolidase
-
Homo sapiens
prolidase
-
Sus scrofa

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.038
-
in 0.05 M Tris-HCl, pH 7.8, 2 mM MnCl2, at 37°C Sus scrofa carmustine
0.082
-
in 0.05 M Tris-HCl, pH 7.8, 2 mM MnCl2, at 37°C Sus scrofa N-[N'-(2-bromoethyl)-N'-nitrosocarbamoyl]-L-proline