Literature summary for 3.4.13.9 extracted from

Liu, G.; Nakayama, K.; Awata, S.; Tang, S.; Kitaoka, N.; Manabe, M.; Kodama, H.
Prolidase isoenzymes in the rat: their organ distribution, developmental change and specific inhibitors (2007), Pediatr. Res., 62, 54-59.

Search for more literature for 3.4.13.9

Inhibitors

Inhibitors	Comment	Organism	Structure
captopril	inhibits isozyme PD I dose-dependently, but shows no inhibition of isozyme PD II at 0.1 mM	Rattus norvegicus
N-Benzyloxycarbonyl-L-proline	inhibits isozyme PD I dose-dependently, but shows no inhibition of isozyme PD II at up to 1 mM	Rattus norvegicus
Ni2+	-	Rattus norvegicus
NiCl2	inhibits isozye PD II much more effectively than isozyme PD I	Rattus norvegicus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	required	Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Rattus norvegicus	lack of prolidase I leads to prolidase deficiency, a disease characterized by intractable skin lesions, recurrent respiratory infections, and mental retardation, physiologic roles of prolidase isoenzymes, PD I functions by way of an intestinal peptide carrier, which may be regulated by the uptake of various iminodipeptides, intestinal PD II also participates in absorption of proline and other amino acids early in life, overview	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	male Wistar rats, isozymes PD I and PD II	-

Purification (Commentary)

Purification (Comment)	Organism
native isozymes partially from kidney by anion exchange chromatography	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Rattus norvegicus	-
heart	-	Rattus norvegicus	-
kidney	isozymes PD I and PD II, high expression level of isozyme PD I, which is predominantly concentrated in renal cortex, where its activity is 2fold higher than in renal medulla	Rattus norvegicus	-
liver	-	Rattus norvegicus	-
lung	-	Rattus norvegicus	-
additional information	organ distribution and developmental changes of isozymes PD I and PD II, overview	Rattus norvegicus	-
pancreas	-	Rattus norvegicus	-
small intestine	high expression level of isozyme PD I	Rattus norvegicus	-
spleen	-	Rattus norvegicus	-
stomach	-	Rattus norvegicus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Ala-Pro + H2O	preferred substrate of isozyme PD I	Rattus norvegicus	Ala + Pro	-	?
Gly-Pro + H2O	-	Rattus norvegicus	Gly + Pro	-	?
Met-Pro + H2O	preferred substrate of isozyme PD II	Rattus norvegicus	Met + Pro	-	?
additional information	substrate specificity of isozymes, overview	Rattus norvegicus	?	-	?
additional information	lack of prolidase I leads to prolidase deficiency, a disease characterized by intractable skin lesions, recurrent respiratory infections, and mental retardation, physiologic roles of prolidase isoenzymes, PD I functions by way of an intestinal peptide carrier, which may be regulated by the uptake of various iminodipeptides, intestinal PD II also participates in absorption of proline and other amino acids early in life, overview	Rattus norvegicus	?	-	?
Ser-Pro + H2O	preferred substrate of isozyme PD I	Rattus norvegicus	Ser + Pro	-	?
Val-Pro + H2O	-	Rattus norvegicus	Val + Pro	-	?

Synonyms

Synonyms	Comment	Organism
prolidase I	-	Rattus norvegicus
prolidase II	-	Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Rattus norvegicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.8	-	assay at	Rattus norvegicus

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.005	-	isozyme PD I, pH 7.8, 37°C	Rattus norvegicus	N-Benzyloxycarbonyl-L-proline
0.05	-	isozyme PD I, pH 7.8, 37°C	Rattus norvegicus	captopril
0.05	-	isozyme PD II, pH 7.8, 37°C	Rattus norvegicus	Ni2+

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Release 2023.1 (January 2023)