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Literature summary for 3.4.13.9 extracted from

  • Lee, H.J.; Chen, Y.S.; Chou, C.Y.; Chien, C.H.; Lin, C.H.; Chang, G.G.; Chen, X.
    Investigation of the dimer interface and substrate specificity of prolyl dipeptidase DPP8 (2006), J. Biol. Chem., 281, 38653-38662.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged wild-type and mutant enzymes in HEK-293T cells, expression in Spodoptera frugiperda Sf9 cells via baculovirus infection system Homo sapiens

Protein Variants

Protein Variants Comment Organism
F822A site-directed mutagenesis, the mutant enzyme shows reduced activity compared to the wild-type enzyme Homo sapiens
H859A site-directed mutagenesis, the mutant enzyme shows reduced activity compared to the wild-type enzyme Homo sapiens
V833A site-directed mutagenesis, the mutant enzyme shows reduced activity compared to the wild-type enzyme Homo sapiens
Y844A site-directed mutagenesis, the mutant enzyme shows reduced activity compared to the wild-type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics and substrate specificity of wild-type and mutant enzymes, overview Homo sapiens
0.127
-
Ala-Pro pH 7.8, 37°C, recombinant His-tagged wild-type enzyme Homo sapiens
1.495
-
Ala-Pro pH 7.8, 37°C, recombinant His-tagged mutant F822A Homo sapiens
2.219
-
Ala-Pro pH 7.8, 37°C, recombinant His-tagged mutant V833A Homo sapiens
2.397
-
Ala-Pro pH 7.8, 37°C, recombinant His-tagged mutant Y844A Homo sapiens
2.72
-
Ala-Pro pH 7.8, 37°C, recombinant His-tagged mutant H859A Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Homo sapiens 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
hydrodynamic properties of wild-type and mutant DPP8s Homo sapiens
105000
-
2 * 105000, recombinant His-tagged enzyme, SDS-PAGE Homo sapiens
210000
-
analytical ultracentrifugation Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from HEK-293T cells by nickel affinity and anion exchange chromatography, followed by gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Pro + H2O
-
Homo sapiens Ala + Pro
-
?
Arg-Pro + H2O preferred substrate Homo sapiens Arg + Pro
-
?
Asp-Pro + H2O
-
Homo sapiens Asp + Pro
-
?
Gly-Pro + H2O
-
Homo sapiens Gly + Pro
-
?
Leu-Pro + H2O
-
Homo sapiens Leu + Pro
-
?
Lys-Pro + H2O
-
Homo sapiens Lys + Pro
-
?
Met-Pro + H2O high activity Homo sapiens Met + Pro
-
?
additional information substrate specificity of wild-type and mutant enzymes, overview Homo sapiens ?
-
?
Ser-Pro + H2O
-
Homo sapiens Ser + Pro
-
?

Subunits

Subunits Comment Organism
dimer 2 * 105000, recombinant His-tagged enzyme, SDS-PAGE Homo sapiens
More hydrodynamic properties of wild-type and mutant DPP8s, overview Homo sapiens

Synonyms

Synonyms Comment Organism
DPP8
-
Homo sapiens
More DPP8 belongs to the family of prolyl dipeptidases Homo sapiens
prolyl dipeptidase
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
Ala-Pro pH 7.8, 37°C, recombinant His-tagged mutants F822A and Y844A Homo sapiens
1
-
Ala-Pro pH 7.8, 37°C, recombinant His-tagged mutant V833A Homo sapiens
8
-
Ala-Pro pH 7.8, 37°C, recombinant His-tagged mutant H859A Homo sapiens
22
-
Ala-Pro pH 7.8, 37°C, recombinant His-tagged wild-type enzyme Homo sapiens