Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
Met-Pro | native prolidase | Pyrococcus furiosus | |
3 | - |
Leu-Pro | native prolidase | Pyrococcus furiosus | |
3.3 | - |
Met-Pro | recombinant prolidase | Pyrococcus furiosus | |
4.2 | - |
Val-Pro | native prolidase | Pyrococcus furiosus | |
8.3 | - |
Ala-Pro | native prolidase | Pyrococcus furiosus | |
20 | - |
Phe-Pro | native prolidase | Pyrococcus furiosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | requires Co2+ in addition to one cobalt atom bound per subunit | Pyrococcus furiosus | |
Mn2+ | Co2+ can be replaced by Mn2+ | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
2 * 42000, SDS-PAGE | Pyrococcus furiosus |
100000 | - |
gel filtration | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native and recombinant form | Pyrococcus furiosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
630 | - |
native enzyme | Pyrococcus furiosus |
1360 | - |
recombinant enzyme | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala-Pro + H2O | - |
Pyrococcus furiosus | L-Ala + Pro | - |
? | |
L-Phe-Pro + H2O | - |
Pyrococcus furiosus | L-Phe + Pro | - |
? | |
L-Val-Pro + H2O | - |
Pyrococcus furiosus | L-Val + Pro | - |
? | |
Leu-Pro + H2O | - |
Pyrococcus furiosus | Leu + Pro | - |
? | |
Met-Pro + H2O | - |
Pyrococcus furiosus | Met + Pro | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42000, SDS-PAGE | Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
substrate Met-Pro | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
substrate Met-Pro | Pyrococcus furiosus |