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Literature summary for 3.4.13.22 extracted from
- Extraction of recombinant protein from Escherichia coli by using a novel cell autolysis activity of VanX (2013), Anal. Biochem., 439, 212-217.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | novel cell breakage method based on VanX. The D-Ala-D-Ala dipeptidase encoded in a vancomycin-resistant VanA gene cluster, exhibits a strong cell lysis activity when expressed in isolation in Escherichia coli. Coexpression of VanX with the target protein causes cell autolysis and release of the cellular content into the culture medium. Application of this strategy for two model proteins, a green fluorescent protein variant and Gaussia luciferase, and optimization of the autolysis conditions and coexpression vectors shows that the fluorescence activity of green fluorescent protein variant collected from the medium is identical to that of green fluorescent protein variant purified by conventional methods. Cell breakage by VanX-mediated autolysis is very simple to implement and will efficiently complement traditional methods | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| VanX | - |
Escherichia coli |
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Release 2023.1 (January 2023)
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