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Literature summary for 3.4.13.22 extracted from

  • Bussiere, D.E.; Pratt, S.D.; Katz, L.; Severin, J.M.; Holzman, T.; Park, C.H.
    The structure of VanX reveals a novel amino-dipeptidase involved in mediating transposon-based vancomycin resistance (1998), Mol. Cell, 2, 75-84.
    View publication on PubMed
Search for more literature for 3.4.13.22

Application

Application Comment Organism
medicine key drug target in circumventing clinical vancomycin resistance Enterococcus faecium

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme-D-Ala complex, enzyme-D-Ala-D-Ala complex, and enzyme in complex with phosphonate and phosphinate transition-state analogue inhibitors, complexsitting drops, 10 mg/ml protein, plus equal volume of precipitant solution: 0.25 M ammonium sulfate, 25% w/v polyethylene glycol monomethyl ester 5000, 0.1 M MES, 1 mM ZnCl2, X-ray diffraction at 2.1 A resolution structure determination and analysis Enterococcus faecium

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Enterococcus faecium
D-3-[(1-aminoethyl)phosphinyl]-D-2-methylpropionic acid phosphinate analogue of the proposed tetrahedral intermediate of the hydrolysis reaction Enterococcus faecium
D-3-[(1-aminoethyl)phosphonyl]-D-2-methylpropionic acid phosphonate analogue of the proposed tetrahedral intermediate of the hydrolysis reaction Enterococcus faecium

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ dependent on Enterococcus faecium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-Ala-D-Ala + H2O Enterococcus faecium
-
 D-Ala + D-Ala
-
 ?

Organism

Organism UniProt Comment Textmining
Enterococcus faecium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Enterococcus faecium

Reaction

Reaction Comment Organism Reaction ID
D-Ala-D-Ala + H2O = 2 D-Ala active site structure, substrate binding and mechanism Enterococcus faecium
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Ala-D-Ala + H2O
-
 Enterococcus faecium D-Ala + D-Ala
-
 ?

Subunits

Subunits Comment Organism
More monomer secondary structure Enterococcus faecium

Synonyms

Synonyms Comment Organism
D-alanyl-D-alanine dipeptidase
-
 Enterococcus faecium
M15.011 Merops-ID Enterococcus faecium
VanX
-
 Enterococcus faecium