Protein Variants | Comment | Organism |
---|---|---|
N89H/Q153E/L205R | 47fold increase in enzyme activity, increased stability | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.26 | - |
L-Asp-4-nitroanilide | mutant N89H/Q153E/L205R | Salmonella enterica subsp. enterica serovar Typhimurium | |
3.98 | - |
L-Asp-4-nitroanilide | wild-type | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Asp-4-nitroanilide + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | L-Asp + 4-nitroaniline | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
30 min, 25% residual activity for wild-type, 80% residual activity for mutant N89H/Q153E/L205R | Salmonella enterica subsp. enterica serovar Typhimurium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
L-Asp-4-nitroanilide | wild-type | Salmonella enterica subsp. enterica serovar Typhimurium | |
5.83 | - |
L-Asp-4-nitroanilide | mutant N89H/Q153E/L205R | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
mutant N89H/Q153E/L205R | Salmonella enterica subsp. enterica serovar Typhimurium |
7.2 | - |
wild-type | Salmonella enterica subsp. enterica serovar Typhimurium |