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Literature summary for 3.4.13.19 extracted from

  • Campbell, B.J.
    Renal dipeptidase (1970), Methods Enzymol., 19, 722-729.
No PubMed abstract available

General Stability

General Stability Organism
extended dialysis against nonchelating buffers does not result in loss of activity Sus scrofa
no loss of activity upon lyophilization Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Sus scrofa
acetate
-
Sus scrofa
Br-
-
Sus scrofa
Cd2+
-
Sus scrofa
Cl-
-
Sus scrofa
CN-
-
Sus scrofa
F-
-
Sus scrofa
HCO3-
-
Sus scrofa
I-
-
Sus scrofa
additional information inhibition by monovalent cations in decreasing order CN-, SCN-, N3-, I-, NO3-, HCO3-, Br-, OAc-, Cl-, F- Sus scrofa
N3-
-
Sus scrofa
Ni2+
-
Sus scrofa
NO3-
-
Sus scrofa
nucleotides
-
Sus scrofa
phosphate
-
Sus scrofa
SCN-
-
Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
glycyldehydrophenylalanine
-
Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates Sus scrofa
Mn2+ activates Sus scrofa
Zinc contains 1 mol of zinc per mol of enzyme Sus scrofa
Zinc no absolute specificity for Zn2+ activation Sus scrofa
Zinc contains 1 mol of zinc per mol of enzyme of MW 47200 Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47200
-
approach to equilibrium measurement Sus scrofa
87000 93000 sedimentation equilibrium measurement, gel filtration Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
hog
-

Purification (Commentary)

Purification (Comment) Organism
-
Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Sus scrofa

Storage Stability

Storage Stability Organism
frozen, in dilute solution, 0.13 mg/ml Tris-HCl buffer, pH 8, loses two-third of its activity after 6 months Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly-D-Phe + H2O
-
Sus scrofa Gly + D-Phe
-
?
Gly-Gly + H2O
-
Sus scrofa Gly + Gly
-
?
glycyldehydrophenylalanine + H2O
-
Sus scrofa ?
-
?
L-Ala-Gly + H2O
-
Sus scrofa L-Ala + Gly
-
?
additional information broad specificity Sus scrofa ?
-
?
additional information no esterase activity Sus scrofa ?
-
?
additional information no hydrolysis of proteins Sus scrofa ?
-
?
additional information no hydrolysis of tripeptides Sus scrofa ?
-
?
additional information no hydrolysis of leucinamide Sus scrofa ?
-
?
additional information no reaction with leucinamide Sus scrofa ?
-
?
additional information requires the N-terminal amino acid of the dipeptide in the L-configuration Sus scrofa ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
glycyldehydrophenylalanine Sus scrofa

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
inactivation below Sus scrofa
8
-
stable at Sus scrofa