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Literature summary for 3.4.13.18 extracted from

  • Wang, W.; Liu, G.; Yamashita, K.; Manabe, M.; Kodama, H.
    Characteristics of prolinase against various iminodipeptides in erythrocyte lysates from a normal human and a patient with prolidase deficiency (2004), Clin. Chem. Lab. Med., 42, 1102-1108.
    View publication on PubMed
Search for more literature for 3.4.13.18

Activating Compound

Activating Compound Comment Organism Structure
Ala prolinase activity in erythrocytes from both the normal control and the patients with prolidase (EC 3.4.13.9) deficiency is activated Homo sapiens
D-Leu enhances activity at concentrations below 20 mM Homo sapiens
D-Val enhances activity at concentrations below 50 mM Homo sapiens
Gly prolinase activity in erythrocytes from both the normal control and the patients with prolidase (EC 3.4.13.9) deficiency is activated Homo sapiens
Ser prolinase activity in erythrocytes from both the normal control and the patients with prolidase (EC 3.4.13.9) deficiency is activated Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
L-leucine strong inhibition of prolinase activity against Pro-Gly regardless wether MnCl2 is present or not Homo sapiens
L-Val strong inhibition of prolinase activity against Pro-Gly regardless wether MnCl2 is present or not Homo sapiens
Mn2+ 0.1 mM, inhibits prolinase activity in normal erythrocytes against Pro-Ala, Pro-Val, Pro-Met, Pro-Asp Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2
-
 Pro-Val pH 7.8, 37°C, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 20 mM Gly Homo sapiens
4.2
-
 Pro-Met pH 7.8, 37°C, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 20 mM Gly Homo sapiens
5
-
 Pro-Met pH 7.8, 37°C, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 0.1 mM MnCl2 and 20 mM Gly Homo sapiens
5.5
-
 Pro-Val pH 7.8, 37°C, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 0.1 mM MnCl2 and 20 mM Gly Homo sapiens
6.8
-
 Pro-Gly pH 7.8, 37°, erythrocyte lysate from healthy patients, activated by 0.1 mM MnCl2 and 20 mM Gly Homo sapiens
7.9
-
 Pro-Val pH 7.8, 37°C, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, without activating compound Homo sapiens
8.1
-
 Pro-Met pH 7.8, 37°C, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, without activating compound Homo sapiens
8.35
-
 Pro-Met pH 7.8, 37°C, erythrocyte lysate from healthy patients, activated by 20 mM Gly Homo sapiens
8.6
-
 Pro-Gly pH 7.8, 37°, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 0.1 mM MnCl2 and 20 mM Gly Homo sapiens
9.7
-
 Pro-Gly pH 7.8, 37°, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 0.1 mM MnCl2 Homo sapiens
9.7
-
 Pro-Met pH 7.8, 37°C, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 0.1 mM MnCl2 Homo sapiens
9.9
-
 Pro-Met pH 7.8, 37°C, erythrocyte lysate from healthy patients, activated by 0.1 mM MnCl2 and 20 mM Gly Homo sapiens
10.1
-
 Pro-Val pH 7.8, 37°C, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 0.1 mM MnCl2 Homo sapiens
13.8
-
 Pro-Gly pH 7.8, 37°, erythrocyte lysate from healthy patients, activated by 0.1 mM MnCl2 Homo sapiens
14.7
-
 Pro-Val pH 7.8, 37°C, erythrocxyte lysate from healthy patients, activated by 20 mM Gly Homo sapiens
15.1
-
 Pro-Gly pH 7.8, 37°, erythrocyte lysate from healthy patients, activated by 20 mM Gly Homo sapiens
16.7
-
 Pro-Gly pH 7.8, 37°, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 20 mM Gly Homo sapiens
17.3
-
 Pro-Val pH 7.8, 37°C, erythrocyte lysate from healthy patients, activated by 0.1 mM MnCl2 and 20 mM Gly Homo sapiens
17.6
-
 Pro-Met pH 7.8, 37°C, erythrocyte lysate from healthy patients, without activating compound Homo sapiens
18.2
-
 Pro-Gly pH 7.8, 37°, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, without activating compound Homo sapiens
19.1
-
 Pro-Met pH 7.8, 37°C, erythrocyte lysate from healthy patients, activated by 0.1 mM MnCl2 Homo sapiens
26
-
 Pro-Val pH 7.8, 37°C, erythrocyte lysate from healthy patients, without activating compound Homo sapiens
26.9
-
 Pro-Gly pH 7.8, 37°, erythrocyte lysate from healthy patients, without activating compound Homo sapiens
28.6
-
 Pro-Val pH 7.8, 37°C, erythrocxyte lysate from healthy patients, activated by 0.1 mM MnCl2 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 0.1 mM, increases prolinase activity in normal erythrocytes against Pro-Gly, Pro-Glu, Pro-Leu, Pro-Ser and Pro-Phe Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte lysate Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Pro-Ala + H2O
-
 Homo sapiens Pro + Ala
-
 ?
Pro-Asp + H2O
-
 Homo sapiens Pro + Asp
-
 ?
Pro-Glu + H2O
-
 Homo sapiens Pro + Glu
-
 ?
Pro-Gly + H2O
-
 Homo sapiens Pro + Gly
-
 ?
Pro-Leu + H2O
-
 Homo sapiens Pro + Leu
-
 ?
Pro-Met + H2O
-
 Homo sapiens Pro + Met
-
 ?
Pro-Phe + H2O
-
 Homo sapiens Pro + Phe
-
 ?
Pro-Ser + H2O
-
 Homo sapiens Pro + Ser
-
 ?
Pro-Val + H2O
-
 Homo sapiens Pro + Val
-
 ?