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Literature summary for 3.4.13.18 extracted from
- Purification and properties of a mouse ascites tumor dipeptidase, a metalloenzyme (1971), J. Biol. Chem., 246, 660-669.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 1,7-phenanthroline has no effect | Mus musculus |  |
| Co2+ | hydrolysis of the relative poor substrates, Pro-Gly and Gly-Gly is activated, whereas that of Ala-Gly is inhibited | Mus musculus | |
| Mn2+ | hydrolysis of the relative poor substrates, Pro-Gly and Gly-Gly is activated, whereas that of Ala-Gly is inhibited | Mus musculus | |
| additional information | inhibition by high substrate concentrations is observed in cases where the Km is low | Mus musculus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
Ala-Leu | - |
Mus musculus | |
| 22 | - |
Gly-L-Ala | - |
Mus musculus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | hydrolysis of the relative poor substrates, Pro-Gly and Gly-Gly is activated, whereas that of Ala-Gly is inhibited | Mus musculus | |
| Mn2+ | hydrolysis of the relative poor substrates, Pro-Gly and Gly-Gly is activated, whereas that of Ala-Gly is inhibited | Mus musculus | |
| Zn2+ | 0.9 gatom of zinc per mol of enzyme | Mus musculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 85000 | - |
gel filtration | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| ascites tumor cell | - |
Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Ala-Ile + H2O | best substrate for the tumor enzyme | Mus musculus | Ala + Ile | - |
? | |
| Ala-Leu + H2O | - |
Mus musculus | Ala + Leu | - |
? | |
| Gly-L-Ala + H2O | - |
Mus musculus | Gly + L-Ala | - |
? | |
| L-Ala-Gly + H2O | - |
Mus musculus | L-Ala + Gly | - |
? | |
| additional information | peptides with small NH2-terminal and bulky nonpolar COOH-terminal R groups are preferred | Mus musculus | ? | - |
? | |
| additional information | only unsubstituted L-alpha-dipeptides | Mus musculus | ? | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 43.3 | - |
Ala-Gly | - |
Mus musculus | |
| 33300 | - |
Ala-Ile | - |
Mus musculus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.3 | - |
- |
Mus musculus |
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