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Literature summary for 3.4.11.B5 extracted from
- Characterization of thermostable deblocking aminopeptidases of archaeon Thermococcus onnurineus NA1 by proteomic and biochemical approaches (2012), J. Microbiol., 50, 792-797.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Thermococcus onnurineus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | - |
Thermococcus onnurineus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.46 | - |
L-methionyl 4-nitroanilide | pH 7.5, 80°C | Thermococcus onnurineus | |
| 2.65 | - |
L-leucyl 4-nitroanilide | pH 7.5, 80°C | Thermococcus onnurineus | |
| 10.9 | - |
L-methionyl 4-nitroanilide | pH 7.5, 80°C | Thermococcus onnurineus | |
| 37.5 | - |
L-leucyl 4-nitroanilide | pH 7.5, 80°C | Thermococcus onnurineus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | activates | Thermococcus onnurineus | |
| Ba2+ | activates, relative activity is about 90% compared to Mn2+ | Thermococcus onnurineus | |
| Co2+ | activates, 0.1 mM Co2+ in assay mixture | Thermococcus onnurineus | |
| Co2+ | activates, relative activity is about 80% compared to Mn2+, 0.1 mM Co2+ in assay mixture | Thermococcus onnurineus | |
| Cu2+ | relative activity is about 30% compared to Mn2+ | Thermococcus onnurineus | |
| Cu2+ | relative activity is about 35% compared to Mg2+ | Thermococcus onnurineus | |
| Mg2+ | activates | Thermococcus onnurineus | |
| Mg2+ | activates, relative activity is about 80% compared to Mn2+ | Thermococcus onnurineus | |
| Mn2+ | activates | Thermococcus onnurineus | |
| Mn2+ | relative activity is about 75% compared to Mg2+ | Thermococcus onnurineus | |
| additional information | metal binding residues are His71, Asp188, Asp 244, and Glu222 | Thermococcus onnurineus | |
| Ni2+ | relative activity is about 40% compared to Mn2+ | Thermococcus onnurineus | |
| Ni2+ | relative activity is about 80% compared to Mg2+ | Thermococcus onnurineus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36470 | - |
4 * 36470, calculated from sequence | Thermococcus onnurineus |
| 37360 | - |
2 * 37360, calculated from sequence | Thermococcus onnurineus |
| 37950 | - |
14 * 37950, calculated from sequence | Thermococcus onnurineus |
| 76100 | - |
MALDI-TOF mas spectrometry | Thermococcus onnurineus |
| 148000 | - |
isoenzyme TNA1-DAP3, MALDI-TOF mass spectrometry | Thermococcus onnurineus |
| 539600 | - |
MALDI-TOF mass spectrometry | Thermococcus onnurineus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermococcus onnurineus | - |
- |
- |
| Thermococcus onnurineus | A7J1X4 | - |
- |
| Thermococcus onnurineus | B6YTG7 | - |
- |
| Thermococcus onnurineus | B6YUS8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermococcus onnurineus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-leucyl 4-nitroanilide + H2O | - |
Thermococcus onnurineus | L-leucine + 4-nitroaniline | - |
? | |
| L-methionyl 4-nitroanilide + H2O | - |
Thermococcus onnurineus | L-methionine + 4-nitroaniline | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 37360, calculated from sequence | Thermococcus onnurineus |
| tetradecamer | 14 * 37950, calculated from sequence | Thermococcus onnurineus |
| tetramer | 4 * 36470, calculated from sequence | Thermococcus onnurineus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TNA1-DAP1 | - |
Thermococcus onnurineus |
| TNA1-DAP2 | - |
Thermococcus onnurineus |
| TNA1-DAP3 | - |
Thermococcus onnurineus |
| TNA1-DAP4 | - |
Thermococcus onnurineus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
assay at | Thermococcus onnurineus |
| 90 | - |
- |
Thermococcus onnurineus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 100 | 60°C: about 60% of maximal activity, 80°C: about 90% of maximal activity, 100°C: about 95% of maximal activity | Thermococcus onnurineus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 96 | - |
half-life: about 2 h | Thermococcus onnurineus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 101 | - |
L-methionyl 4-nitroanilide | pH 7.5, 80°C | Thermococcus onnurineus | |
| 108 | - |
L-leucyl 4-nitroanilide | pH 7.5, 80°C | Thermococcus onnurineus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Thermococcus onnurineus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 41 | - |
L-leucyl 4-nitroanilide | pH 7.5, 80°C | Thermococcus onnurineus | |
| 220 | - |
L-methionyl 4-nitroanilide | pH 7.5, 80°C | Thermococcus onnurineus | |
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