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Literature summary for 3.4.11.B5 extracted from

  • Lee, H.S.; Cho, Y.; Kim, Y.J.; Nam, K.; Lee, J.H.; Kang, S.G.
    Biochemical characterization of deblocking aminopeptidase from hyperthermophilic archaeon Thermococcus onnurineus NA1 (2007), J. Biosci. Bioeng., 104, 188-194.
    View publication on PubMed
Search for more literature for 3.4.11.B5

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermococcus onnurineus

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 0.5 mM Thermococcus onnurineus
EDTA 0.5 mM, complete inhibition Thermococcus onnurineus
Mn2+ 0.5 mM Thermococcus onnurineus
Ni2+ 0.5 mM Thermococcus onnurineus
Zn2+ 0.5 mM, complete inhibition Thermococcus onnurineus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 L-methionyl 4-nitroanilide pH 7.5, 80°C Thermococcus onnurineus
0.8
-
 L-leucyl 4-nitroanilide pH 7.5, 80°C Thermococcus onnurineus
1.1
-
 N-acetyl-L-leucyl 4-nitroanilide pH 7.5, 80°C Thermococcus onnurineus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 0.5 mM Co2+ stimulates activity 4.8fold, the enzyme contains 0.09 mol of Co2+ per mol of monomer Thermococcus onnurineus
NaCl stimulates, optimal cobcentration Thermococcus onnurineus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37900
-
 x * 37900, His-tagged enzyme, SDS-PAGE Thermococcus onnurineus
71000
-
 dimeric form prevalent in presence of 0.1 mM Co2+, gel filtration Thermococcus onnurineus
600000
-
 oligomeric form prevalent in absence of Co2+, gel filtration Thermococcus onnurineus

Organism

Organism UniProt Comment Textmining
Thermococcus onnurineus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermococcus onnurineus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-leucyl 4-nitroanilide + H2O catalytic efficiency for hydrolysis of Leu-4-nitroanilide is 105fold higher than catalytic efficiency for hydrolysis of acetyl-Leu-4-nitroanilide Thermococcus onnurineus L-leucine + 4-nitroaniline
-
 ?
L-methionyl 4-nitroanilide + H2O
-
 Thermococcus onnurineus L-methionine + 4-nitroaniline
-
 ?
N-acetyl-L-leucyl 4-nitroanilide + H2O catalytic efficiency for hydrolysis of Leu-4-nitroanilide is 105fold higher than catalytic efficiency for hydrolysis of acetyl-Leu-4-nitroanilide Thermococcus onnurineus N-acetyl-L-leucine + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 37900, His-tagged enzyme, SDS-PAGE Thermococcus onnurineus

Synonyms

Synonyms Comment Organism
deblocking aminopeptidase ambiguous Thermococcus onnurineus
TNA1-DAP
-
 Thermococcus onnurineus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90 100
-
 Thermococcus onnurineus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70 100 70°C: about 35% of maximal activity, 90-100°C: optimum Thermococcus onnurineus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
96
-
 1 h, t1/2 is less than 10 min in 50 mM HEPES buffer (pH 7.5) in absence of CoCl2, stable for at least 1 h in presence of 0.1 mM CoCl2 Thermococcus onnurineus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.5
-
 N-acetyl-L-leucyl 4-nitroanilide pH 7.5, 80°C Thermococcus onnurineus
10.9
-
 L-methionyl 4-nitroanilide pH 7.5, 80°C Thermococcus onnurineus
37.5
-
 L-leucyl 4-nitroanilide pH 7.5, 80°C Thermococcus onnurineus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Thermococcus onnurineus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8 pH 6.5: about 50% of maximal activity, pH 8.5: about 70% of maximal activity (HEPES buffer), about 40% of maximal activity (Tris-HCl buffer) Thermococcus onnurineus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.45
-
 N-acetyl-L-leucyl 4-nitroanilide pH 7.5, 80°C Thermococcus onnurineus
47
-
 L-leucyl 4-nitroanilide pH 7.5, 80°C Thermococcus onnurineus
55
-
 L-methionyl 4-nitroanilide pH 7.5, 80°C Thermococcus onnurineus