Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus horikoshii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.8 | - |
Ala-Ala-Ala-Ala-Ala | pH 7.5, 85°C | Pyrococcus horikoshii | |
13.2 | - |
Ala-Ala-Ala-Ala | pH 7.5, 85°C | Pyrococcus horikoshii | |
18.2 | - |
Ala-Ala-Ala | pH 7.5, 85°C | Pyrococcus horikoshii | |
39.9 | - |
Ala-Ala | pH 7.5, 85°C | Pyrococcus horikoshii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the enzyme is chelated with one calcium ion per monomer of the protein. Removal of Ca2+ by dialysis for 12 h in 50 mM EDTA does not effect the enzyme activity, suggesting that calcium has no effect on the activity. Replacement of calcium with zinc also shows no effect on the activity | Pyrococcus horikoshii | |
Co2+ | activity is dependent on Co2+. 6fold increase in activity in presence of 0.5 mM Co2+ | Pyrococcus horikoshii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36900 | - |
x * 36900, SDS-PAGE | Pyrococcus horikoshii |
440000 | - |
gel filtration, HPLC | Pyrococcus horikoshii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | O58255 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Ala + H2O | - |
Pyrococcus horikoshii | L-alanine + L-alanine | - |
? | |
Ala-Ala-Ala + H2O | the activity (turnover number) with N-acetyl-Ala-Ala-Ala is 260fold lower compared to the activity with Ala-Ala-Ala | Pyrococcus horikoshii | Ala-Ala + L-alanine | - |
? | |
Ala-Ala-Ala-Ala + H2O | - |
Pyrococcus horikoshii | Ala-Ala-Ala + L-alanine | - |
? | |
Ala-Ala-Ala-Ala-Ala + H2O | - |
Pyrococcus horikoshii | Ala-Ala-Ala-Ala + L-alanine | - |
? | |
additional information | no activity with Ala-Pro-Ala | Pyrococcus horikoshii | ? | - |
? | |
N-acetyl-Ala-Ala-Ala + H2O | the activity (turnover number) with N-acetyl-Ala-Ala-Ala is 260fold lower compared to the activity with Ala-Ala-Ala | Pyrococcus horikoshii | Ala-Ala + N-acetyl-L-alanine | - |
? | |
N-acetyl-L-alanyl 4-nitroanilide + H2O | - |
Pyrococcus horikoshii | N-acetyl-L-alanine + 4-nitroaniline | - |
? | |
N-acetyl-L-leucyl 4-nitroanilide + H2O | - |
Pyrococcus horikoshii | N-acetyl-L-leucine + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | x * 36900, SDS-PAGE | Pyrococcus horikoshii |
Synonyms | Comment | Organism |
---|---|---|
PH0519 | - |
Pyrococcus horikoshii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
- |
Pyrococcus horikoshii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | 98 | 85°C: about 35% of maximal activity, 98°C: about 85% of maximal activity | Pyrococcus horikoshii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
2 h, about 20% loss of activity | Pyrococcus horikoshii |
95 | - |
about 15% loss of activity after 1 h, about 70% loss of activity after 2 h | Pyrococcus horikoshii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
N-acetyl-L-alanyl 4-nitroanilide | pH 7.5, 85°C | Pyrococcus horikoshii | |
0.116 | - |
N-acetyl-L-leucyl 4-nitroanilide | pH 7.5, 85°C | Pyrococcus horikoshii | |
0.18 | - |
Ala-Ala | pH 7.5, 85°C | Pyrococcus horikoshii | |
8.71 | - |
Ala-Ala-Ala | pH 7.5, 85°C | Pyrococcus horikoshii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Pyrococcus horikoshii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.3 | pH 6.5: about 20% of maximal activity, pH 7.0: about 90% of maximal activity, pH 8.3: about 50% of maximal activity | Pyrococcus horikoshii |