Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.11.7 extracted from

  • Rozenfeld, R.; Iturrioz, X.; Okada, M.; Maigret, B.; Llorens-Cortes, C.
    Contribution of molecular modeling and site-directed mutagenesis to the identification of a new residue, glutamate 215, involved in the exopeptidase specificity of aminopeptidase A (2003), Biochemistry, 42, 14785-14793.
    View publication on PubMed
Search for more literature for 3.4.11.7

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in CHO cells Mus musculus

Protein Variants

Protein Variants Comment Organism
E215A the ratio of turnover number to KM-value for the substrate alpha-L-Glu-beta-naphthylamide is 10% of that of the wild-type enzyme. 68fold increase in KI-value for glutamate-thiol, 466fold increase in KI-value for L-glutamate phosphonic acid, 5.8fold increase in Ki-value for D-glutamate phosphonic acid Mus musculus
E215D the ratio of turnover number to KM-value for the substrate alpha-L-Glu-beta-naphthylamide is 7% of that of the wild-type enzyme. 49fold increase in KI-value for glutamate-thiol, 266fold increase in KI-value for L-glutamate phosphonic acid, 2.6fold increase in Ki-value for D-glutamate phosphonic acid Mus musculus
E215Q 1.2fold increase in the ratio of turnover number to KM-value for the substrate alpha-L-Glu-beta-naphthylamide. 2.3fold increase in KI-value for glutamate-thiol, 5.8fold increase in KI-value for L-glutamate phosphonic acid, 8.4fold increase in Ki-value for D-glutamate phosphonic acid Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
D-glutamate phosphonic acid
-
 Mus musculus
glutamate-thiol
-
 Mus musculus
L-glutamate phosphonic acid
-
 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
 alpha-L-Glu-beta-naphthylamide pH 7.4, 37°C, wild-type enzyme Mus musculus
0.87
-
 alpha-L-Glu-beta-naphthylamide pH 7.4, 37°C, mutant enzyme E215Q Mus musculus
1.5
-
 alpha-L-Glu-beta-naphthylamide pH 7.4, 37°C, mutant enzyme E215D Mus musculus
2.8
-
 alpha-L-Glu-beta-naphthylamide pH 7.4, 37°C, mutant enzyme E215A Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane wild-type and mutant enzymes in stably transfected mutant enzymes Mus musculus 5886
-

Organism

Organism UniProt Comment Textmining
Mus musculus P16406
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-L-Glu-beta-naphthylamide + H2O
-
 Mus musculus L-Glu + 2-naphthylamine
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
19
-
 alpha-L-Glu-beta-naphthylamide pH 7.4, 37°C, mutant enzyme E215D Mus musculus
48
-
 alpha-L-Glu-beta-naphthylamide pH 7.4, 37°C, mutant enzyme E215A Mus musculus
270
-
 alpha-L-Glu-beta-naphthylamide pH 7.4, 37°C, wild-type enzyme Mus musculus
1800
-
 alpha-L-Glu-beta-naphthylamide pH 7.4, 37°C, mutant enzyme E215Q Mus musculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000045
-
 L-glutamate phosphonic acid pH 7.4, 37°C, wild-type enzyme Mus musculus
0.000147
-
 glutamate-thiol pH 7.4, 37°C, wild-type enzyme Mus musculus
0.00026
-
 L-glutamate phosphonic acid pH 7.4, 37°C, mutant enzyme E215Q Mus musculus
0.00034
-
 glutamate-thiol pH 7.4, 37°C, mutant enzyme E215Q Mus musculus
0.0019
-
 D-glutamate phosphonic acid pH 7.4, 37°C, wild-type enzyme Mus musculus
0.005
-
 D-glutamate phosphonic acid pH 7.4, 37°C, mutant enzyme E215D Mus musculus
0.0072
-
 glutamate-thiol pH 7.4, 37°C, mutant enzyme E215D Mus musculus
0.01
-
 glutamate-thiol pH 7.4, 37°C, mutant enzyme E215A Mus musculus
0.011
-
 D-glutamate phosphonic acid pH 7.4, 37°C, mutant enzyme E215A Mus musculus
0.012
-
 L-glutamate phosphonic acid pH 7.4, 37°C, mutant enzyme E215D Mus musculus
0.016
-
 D-glutamate phosphonic acid pH 7.4, 37°C, mutant enzyme E215Q Mus musculus
0.021
-
 L-glutamate phosphonic acid pH 7.4, 37°C, mutant enzyme E215A Mus musculus