Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NaF | 0.4 M activates the activity by 20% | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 34% residual activity at 1 mM | Rattus norvegicus | |
actinonin | - |
Rattus norvegicus | |
Aprotinin | 34% residual activity at 0.25 mg/ml | Rattus norvegicus | |
arphamemine B | - |
Rattus norvegicus | |
bestatin | highly sensitive to bestatin | Rattus norvegicus | |
Ca2+ | 19% inhibition at 1 mM | Rattus norvegicus | |
Cd2+ | complete inhibition at 1 mM | Rattus norvegicus | |
Co2+ | 76% inhibition at 1 mM | Rattus norvegicus | |
Cu2+ | complete inhibition at 1 mM | Rattus norvegicus | |
E-64 | 99% residual activity at 0.1 mM | Rattus norvegicus | |
EDTA | 61% residual activity at 1 mM | Rattus norvegicus | |
EGTA | complete inhibition at 1 mM | Rattus norvegicus | |
Hg2+ | complete inhibition at 1 mM | Rattus norvegicus | |
Leucine hydroxamate | - |
Rattus norvegicus | |
leucinthiol | - |
Rattus norvegicus | |
Mn2+ | 82% inhibition at 1 mM | Rattus norvegicus | |
additional information | insensitive to L-Arg-hydroxamate, L-Lys-hydroxamate, puromycin, and amastatin | Rattus norvegicus | |
Na+ | NaCl (0.1-100 mM) has an opposite effect on the EGTA-treated KAP apo-enzyme, it inhibits 13% at 0.1 mM and 100% at 100 mM | Rattus norvegicus | |
Ni2+ | complete inhibition at 1 mM | Rattus norvegicus | |
Pb2+ | 84% inhibition at 1 mM | Rattus norvegicus | |
pepstatin | 93% residual activity at 0.1 mM | Rattus norvegicus | |
Phenylmethanesulfonylfluoride | 45% residual activity at 0.5 mM | Rattus norvegicus | |
Zn2+ | 80% inhibition at 1 mM | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.333 | - |
L-Lys-2-naphthylamide | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
membrane | synaptic membrane | Rattus norvegicus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 0.4 M activates the activity by 55% | Rattus norvegicus | |
Li+ | 0.4 M activates the activity by 45% | Rattus norvegicus | |
Na+ | 0.4 M activates the activity by 190% | Rattus norvegicus | |
Zn2+ | Zn2+-exopeptidase | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Toyopearl DEAE-650 column chromatography, Mono Q column chromatography, and DEAE-650 column chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | - |
Rattus norvegicus | - |
brain | - |
Rattus norvegicus | - |
cerebellum | - |
Rattus norvegicus | - |
cerebral cortex | - |
Rattus norvegicus | - |
hippocampus | - |
Rattus norvegicus | - |
hypothalamus | - |
Rattus norvegicus | - |
intestine | - |
Rattus norvegicus | - |
kidney | - |
Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
medulla oblongata | - |
Rattus norvegicus | - |
midbrain | - |
Rattus norvegicus | - |
muscle | - |
Rattus norvegicus | - |
pancreas | - |
Rattus norvegicus | - |
pituitary gland | - |
Rattus norvegicus | - |
serum | - |
Rattus norvegicus | - |
spleen | - |
Rattus norvegicus | - |
testis | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gly-2-naphthylamide + H2O | 1% activity compared to L-Lys-2-naphthylamide | Rattus norvegicus | Gly + 2-naphthylamine | - |
? | |
Gly-L-Pro-2-naphthylamide + H2O | 1% activity compared to L-Lys-2-naphthylamide | Rattus norvegicus | Gly-L-Pro + 2-naphthylamine | - |
? | |
L-Ala-2-naphthylamide + H2O | 83% activity compared to L-Lys-2-naphthylamide | Rattus norvegicus | L-Ala + 2-naphthylamine | - |
? | |
L-Arg-2-naphthylamide + H2O | 17% activity compared to L-Lys-2-naphthylamide | Rattus norvegicus | L-Arg + 2-naphthylamine | - |
? | |
L-beta-Asn-2-naphthylamide + H2O | 9% activity compared to L-Lys-2-naphthylamide | Rattus norvegicus | L-beta-Asn + 2-naphthylamine | - |
? | |
L-Cys-2-naphthylamide + H2O | 1% activity compared to L-Lys-2-naphthylamide | Rattus norvegicus | L-Cys + 2-naphthylamine | - |
? | |
L-Gly-L-Arg-2-naphthylamide + H2O | 250% activity compared to L-Lys-2-naphthylamide | Rattus norvegicus | L-Gly-L-Arg + 2-naphthylamine | - |
? | |
L-His-2-naphthylamide + H2O | 15% activity compared to L-Lys-2-naphthylamide | Rattus norvegicus | L-His + 2-naphthylamine | - |
? | |
L-Leu-2-naphthylamide + H2O | 31% activity compared to L-Lys-2-naphthylamide | Rattus norvegicus | L-Leu + 2-naphthylamine | - |
? | |
L-Lys-2-naphthylamide + H2O | 100% activity | Rattus norvegicus | L-Lys + 2-naphthylamine | - |
? | |
L-Lys-L-Ala-2-naphthylamide + H2O | 7% activity compared to L-Lys-2-naphthylamide | Rattus norvegicus | L-Lys-L-Ala + 2-naphthylamine | - |
? | |
L-Met-2-naphthylamide + H2O | 2% activity compared to L-Lys-2-naphthylamide | Rattus norvegicus | L-Met + 2-naphthylamine | - |
? | |
L-Tyr-2-naphthylamide + H2O | 62% activity compared to L-Lys-2-naphthylamide | Rattus norvegicus | L-Tyr + 2-naphthylamine | - |
? | |
additional information | no activity with L-alpha-Asn-2-naphthylamide, L-Glu-2-naphthylamide, L-Ile-2-naphthylamide, L-Phe-2-naphthylamide, L-Pro-2-naphthylamide, L-Ser-2-naphthylamide, L-Thr-2-naphthylamide, L-Trp-2-naphthylamide, L-Val-2-naphthylamide, and L-Arg-L-Arg-2-naphthylamide | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aminopeptidase B | - |
Rattus norvegicus |
APB | - |
Rattus norvegicus |
KAP | - |
Rattus norvegicus |
lysine-specific aminopeptidase | - |
Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | - |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00021 | - |
bestatin | at 37°C in Bicine buffer complemented with 0.2 M NaCl | Rattus norvegicus | |
0.00054 | - |
leucinthiol | at 37°C in Bicine buffer complemented with 0.2 M NaCl | Rattus norvegicus | |
0.0125 | - |
arphamemine B | at 37°C in Bicine buffer complemented with 0.2 M NaCl | Rattus norvegicus | |
0.02 | - |
actinonin | at 37°C in Bicine buffer complemented with 0.2 M NaCl | Rattus norvegicus | |
0.024 | - |
Leucine hydroxamate | at 37°C in Bicine buffer complemented with 0.2 M NaCl | Rattus norvegicus |