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Literature summary for 3.4.11.6 extracted from

  • Hirose, J.; Ohsaki, T.; Nishimoto, N.; Matuoka, S.; Hiromoto, T.; Yoshida, T.; Minoura, T.; Iwamoto, H.; Fukasawa, K.M.
    Characterization of the metal-binding site in aminopeptidase B (2006), Biol. Pharm. Bull., 29, 2378-2382.
    View publication on PubMed
Search for more literature for 3.4.11.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21 Rattus norvegicus
into the pGEX-4T-3 vector for expression in Escherichia coli BL21 cells Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Rattus norvegicus
2,6-pyridinedicarboxylate
-
 Rattus norvegicus
bipyridine
-
 Rattus norvegicus
EDTA
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000183
-
 L-Arg-2-naphthylamide metal-substituted enzyme form Co(II)-Ap-B, at pH 7.4 and 25°C Rattus norvegicus
0.000267
-
 L-Arg-2-naphthylamide recombinant Ap-B, at pH 7.4 and 25°C Rattus norvegicus
0.183
-
 Arg-beta-naphthylamide Co(II)-Ap-B Rattus norvegicus
0.267
-
 Arg-beta-naphthylamide Ap-B Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+
-
 Rattus norvegicus
Co2+ the metal-substituted derivative Co(II)-Ap-B contains almost 1 mol of cobalt(II) ions molecule Rattus norvegicus
Cu2+
-
 Rattus norvegicus
Cu2+ the metal-substituted derivative Cu(II)-Ap-B contains almost 1 mol of copper(II) ions per molecule Rattus norvegicus
Zn2+
-
 Rattus norvegicus
Zn2+ Ap-B contains 1 mol of Zn2+ ions per molecule Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione-Sepharose 4B column chromatography and Q-Sepharose column chromatography Rattus norvegicus
using a glutathione-Sepharose 4B column, cleaving of the fusion-enzyme by thrombin, using a benzamidine-Sepharose and a Q-Sepharose column Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
duodenum
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.1
-
 metal-substituted enzyme form Cu(II)-Ap-B, using L-Arg-2-naphthylamide as a substrate, at pH 7.4 and 25°C Rattus norvegicus
0.1
-
 Cu(II)-Ap-B Rattus norvegicus
9.9
-
 metal-substituted enzyme form Co(II)-Ap-B, using L-Arg-2-naphthylamide as a substrate, at pH 7.4 and 25°C Rattus norvegicus
9.9
-
 Co(II)-Ap-B Rattus norvegicus
12.5
-
 recombinant Ap-B, using L-Arg-2-naphthylamide as a substrate, at pH 7.4 and 25°C Rattus norvegicus
12.5
-
 Ap-B Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Arg-beta-naphthylamide + H2O substrate used in the activity assay Rattus norvegicus L-arginine + 2-naphthylamine
-
 ?
L-Arg-2-naphthylamide + H2O
-
 Rattus norvegicus L-Arg + 2-naphthylamine
-
 ?

Synonyms

Synonyms Comment Organism
aminopeptidase B
-
 Rattus norvegicus
aminopeptidase-B
-
 Rattus norvegicus
Ap-B
-
 Rattus norvegicus
Co(II)-Ap-B metal-substituted derivative of Ap-B Rattus norvegicus
Cu(II)-Ap-B metal-substituted derivative of Ap-B Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 activity assay Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
53.1
-
 L-Arg-2-naphthylamide recombinant Ap-B, at pH 7.4 and 25°C Rattus norvegicus
53.1
-
 Arg-beta-naphthylamide Ap-B Rattus norvegicus
69.47
-
 L-Arg-2-naphthylamide metal-substituted enzyme form Co(II)-Ap-B, at pH 7.4 and 25°C Rattus norvegicus
69.5
-
 Arg-beta-naphthylamide Co(II)-Ap-B Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 activity assay Rattus norvegicus