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Literature summary for 3.4.11.6 extracted from

  • Foulon, T.; Cadel, S.; Piesse, C.; Cohen, P.
    Aminopeptidase B (2004), Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds) Academic Press, 1, 328-332.
No PubMed abstract available
Search for more literature for 3.4.11.6

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme is up-regulated during long-term activation of normal and lymphoma T-cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline reversible by Zn2+ Rattus norvegicus
4-chloromercuribenzoate
-
 Rattus norvegicus
amastatin
-
 Rattus norvegicus
arphamenine A
-
 Rattus norvegicus
Arphamenine B
-
 Rattus norvegicus
bestatin
-
 Rattus norvegicus
DTT
-
 Rattus norvegicus
EDTA
-
 Rattus norvegicus
EGTA
-
 Rattus norvegicus
additional information no inhibition by PMSF, pepstatin, and aprotinin Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 Arg-Lys-somatostatin-14
-
 Rattus norvegicus
0.014
-
 Arg-alpha-atrial natriuretic factor1-20
-
 Rattus norvegicus
0.02
-
 L-Arg-2-naphthylamide
-
 Rattus norvegicus
0.036
-
 L-Lys-2-naphthylamide
-
 Rattus norvegicus
0.058
-
 Arg-neurokinin A
-
 Rattus norvegicus
0.111
-
 Arg-Leu-enkephalin
-
 Rattus norvegicus
0.125
-
 Arg-Met-enkephalin
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
acrosome of germinal cells, proacrosmic granule of round spermatids Rattus norvegicus
-
-
cytoplasm of late spermatids Rattus norvegicus 5737
-
extracellular the enzyme is secreted, in PC-12 cells the active enzyme is associated to the external surface of the plassma mamabrane Rattus norvegicus
-
-
Golgi apparatus of germinal cells Rattus norvegicus 5794
-
secondary lysosome of late spermatids Rattus norvegicus 5767
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cl- activates at physiological concentrations Mus musculus
Cl- activates at physiological concentrations Homo sapiens
Cl- activates at physiological concentrations around 150 mM, dependent on the target peptide Rattus norvegicus
Zn2+ zinc-metallopeptidase Mus musculus
Zn2+ zinc-metallopeptidase Homo sapiens
Zn2+ dependent on, zinc-metallopeptidase, the enzyme contains the binding motif HEXXHX18E Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72300
-
 1 * 95500, liver enzyme, 1 * 72300, testis enzyme Rattus norvegicus
95500
-
 1 * 95500, liver enzyme, 1 * 72300, testis enzyme Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus the enzyme probably is involved in the final stages of peptide and protein precursor processing and maturation mechanisms via the exopeptidase pathway and thereby in some inflammatory processes and tumour development, the enzyme from testis preferably removes Arg and/or Lys residues from the N-terminus of various peptides ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-
Rattus norvegicus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
release of N-terminal Arg and Lys from oligopeptides when P1' is not Pro. Also acts on arylamides of Arg and Lys exopeptidase, the enzyme is strictly specific for the removal of N-terminal basic residues from peptides and proteins, no activity with substrate possessing Pro at the P1 position Rattus norvegicus
a

Source Tissue

Source Tissue Comment Organism Textmining
BE(2)-M17 cell neuroblastoma cell line Homo sapiens
-
epididymis
-
 Rattus norvegicus
-
GH3 cell pituitary tumor-derived cell line Rattus norvegicus
-
heart
-
 Rattus norvegicus
-
JURKAT cell T-lymphoma cell line Homo sapiens
-
K-562 cell chronic myeloid leukemia cell line Homo sapiens
-
kidney
-
 Rattus norvegicus
-
large intestine
-
 Rattus norvegicus
-
Leydig cell
-
 Rattus norvegicus
-
liver
-
 Rattus norvegicus
-
lung
-
 Rattus norvegicus
-
MOLT-4 cell T-cell line Homo sapiens
-
additional information the enzyme is up-regulated during long-term activation of normal and lymphoma T-cells Homo sapiens
-
Mrc5 cell lung cell line Homo sapiens
-
muscle
-
 Rattus norvegicus
-
pancreas
-
 Rattus norvegicus
-
PC-12 cell adrenal pheochromocytoma cell line Rattus norvegicus
-
pituitary gland
-
 Rattus norvegicus
-
pituitary gland cell line primary pituitary gland cell line Rattus norvegicus
-
small intestine
-
 Rattus norvegicus
-
spermatozoon
-
 Rattus norvegicus
-
spleen
-
 Rattus norvegicus
-
T-lymphocyte
-
 Homo sapiens
-
testis highest enzyme activity, mainly expressed in late spermatids during maturation Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Arg-alpha-atrial natriuretic factor1-20 + H2O
-
 Rattus norvegicus Arg + alpha-atrial natriuretic factor1-20
-
 ?
Arg-Leu-enkephalin + H2O
-
 Rattus norvegicus Arg + Leu-enkephalin
-
 ?
Arg-Lys-somatostatin-14 + H2O
-
 Rattus norvegicus Arg-Lys + somatostatin-14
-
 ?
Arg-Met-enkephalin + H2O
-
 Rattus norvegicus Arg + Met-enkephalin
-
 ?
Arg-neurokinin A + H2O
-
 Rattus norvegicus Arg + neurokinin A
-
 ?
kallidin-10 + H2O
-
 Rattus norvegicus Lys + bradykinin
-
 ?
L-Arg-2-naphthylamide + H2O
-
 Rattus norvegicus L-Arg + 2-naphthylamine
-
 ?
L-Lys-2-naphthylamide + H2O
-
 Rattus norvegicus L-Lys + 2-naphthylamine
-
 ?
leukotriene A4 + H2O weak in vitro activity Rattus norvegicus L-Arg + leukotriene B4
-
 ?
additional information the enzyme probably is involved in the final stages of peptide and protein precursor processing and maturation mechanisms via the exopeptidase pathway and thereby in some inflammatory processes and tumour development, the enzyme from testis preferably removes Arg and/or Lys residues from the N-terminus of various peptides Rattus norvegicus ?
-
 ?
additional information the enzyme is strictly specific for the removal of N-terminal basic residues from peptides and proteins Mus musculus ?
-
 ?
additional information the enzyme is strictly specific for the removal of N-terminal basic residues from peptides and proteins Homo sapiens ?
-
 ?
additional information the enzyme is strictly specific for the removal of N-terminal basic residues from peptides and proteins, no activity with bradykinin, no activity with substrate possessing Pro at the P1 position Rattus norvegicus ?
-
 ?
thymopentin + H2O
-
 Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 95500, liver enzyme, 1 * 72300, testis enzyme Rattus norvegicus

Synonyms

Synonyms Comment Organism
Ap-B
-
 Rattus norvegicus
arginyl aminopeptidase
-
 Rattus norvegicus
arylamidase
-
 Rattus norvegicus
Cl--activated arinine aminopeptidase
-
 Rattus norvegicus
cytosol aminopeptidase IV
-
 Rattus norvegicus
More the enzyme belongs to the M1 peptidase family Mus musculus
More the enzyme belongs to the M1 peptidase family Homo sapiens
More the enzyme belongs to the M1 peptidase family, and is distinct from the leukotriene A4 hydrolase, bleomycin hydrolase, and puromycin-sensitive aminopeptidase Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 broad activity range Rattus norvegicus

pI Value

Organism Comment pI Value Maximum pI Value
Rattus norvegicus experimental
-
 4.9
Rattus norvegicus theoretical
-
 5.83