Activating Compound | Comment | Organism | Structure |
---|---|---|---|
E64 | slight activation at 0.01-0.05 mM | Ustilago maydis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Ustilago maydis | |
bestatin | - |
Ustilago maydis | |
Ca2+ | slight inhibition at 1 mM | Ustilago maydis | |
Co2+ | strong inhibition at 1 mM | Ustilago maydis | |
Cu2+ | complete inhibition at 1 mM | Ustilago maydis | |
EDTA | - |
Ustilago maydis | |
Hg2+ | complete inhibition at 1 mM | Ustilago maydis | |
leupeptin | - |
Ustilago maydis | |
Mg2+ | slight inhibition at 1 mM | Ustilago maydis | |
Mn2+ | strong inhibition at 1 mM | Ustilago maydis | |
additional information | no inhibition by pepstatin | Ustilago maydis | |
Ni2+ | strong inhibition at 1 mM | Ustilago maydis | |
pefabloc | - |
Ustilago maydis | |
PMSF | - |
Ustilago maydis | |
Zn2+ | complete inhibition at 1 mM | Ustilago maydis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0544 | - |
L-Lys-4-nitroanilide | pH 7.5, 37°C | Ustilago maydis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no activation by metal ions, no metallopeptidase | Ustilago maydis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
2 * 58000, SDS-PAGE | Ustilago maydis |
110000 | - |
gel filtration | Ustilago maydis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ustilago maydis | - |
haploid fungus, strain FB1 | - |
Purification (Comment) | Organism |
---|---|
native enzyme 69.3fold by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, and gel filtration to homogeneity | Ustilago maydis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.63 | - |
purified enzyme | Ustilago maydis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala-4-nitroanilide + H2O | - |
Ustilago maydis | L-Ala + 4-nitroaniline | - |
? | |
L-Arg-4-nitroanilide + H2O | - |
Ustilago maydis | L-Arg + 4-nitroaniline | - |
? | |
L-Leu-4-nitroanilide + H2O | - |
Ustilago maydis | L-Leu + 4-nitroaniline | - |
? | |
L-Lys-4-nitroanilide + H2O | - |
Ustilago maydis | L-Lys + 4-nitroaniline | - |
? | |
additional information | substrate specificity, the enzyme prefers Arg or Lys in N-terminal position, no activity with dipeptide-4-nitroanilides, overview | Ustilago maydis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 58000, SDS-PAGE | Ustilago maydis |
Synonyms | Comment | Organism |
---|---|---|
pumAPE | - |
Ustilago maydis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Ustilago maydis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 80 | - |
Ustilago maydis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 88 | 1 h, loss of activity | Ustilago maydis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Ustilago maydis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | narrow pH-optimum | Ustilago maydis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | purified enzyme, 4°C, 12 h, stable | Ustilago maydis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Ustilago maydis | isoelectric focusing | - |
5.1 |