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Literature summary for 3.4.11.6 extracted from

  • Mercado-Flores, Y.; Noriega-Reyes, Y.; Ramirez-Zavala, B.; Hernandez-Rodriguez, C.; Villa-Tanaca, L.
    Purification and characterization of aminopeptidase (pumAPE) from Ustilago maydis (2004), FEMS Microbiol. Lett., 234, 247-253.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
E64 slight activation at 0.01-0.05 mM Ustilago maydis

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Ustilago maydis
bestatin
-
Ustilago maydis
Ca2+ slight inhibition at 1 mM Ustilago maydis
Co2+ strong inhibition at 1 mM Ustilago maydis
Cu2+ complete inhibition at 1 mM Ustilago maydis
EDTA
-
Ustilago maydis
Hg2+ complete inhibition at 1 mM Ustilago maydis
leupeptin
-
Ustilago maydis
Mg2+ slight inhibition at 1 mM Ustilago maydis
Mn2+ strong inhibition at 1 mM Ustilago maydis
additional information no inhibition by pepstatin Ustilago maydis
Ni2+ strong inhibition at 1 mM Ustilago maydis
pefabloc
-
Ustilago maydis
PMSF
-
Ustilago maydis
Zn2+ complete inhibition at 1 mM Ustilago maydis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0544
-
L-Lys-4-nitroanilide pH 7.5, 37°C Ustilago maydis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no activation by metal ions, no metallopeptidase Ustilago maydis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
2 * 58000, SDS-PAGE Ustilago maydis
110000
-
gel filtration Ustilago maydis

Organism

Organism UniProt Comment Textmining
Ustilago maydis
-
haploid fungus, strain FB1
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 69.3fold by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, and gel filtration to homogeneity Ustilago maydis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.63
-
purified enzyme Ustilago maydis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala-4-nitroanilide + H2O
-
Ustilago maydis L-Ala + 4-nitroaniline
-
?
L-Arg-4-nitroanilide + H2O
-
Ustilago maydis L-Arg + 4-nitroaniline
-
?
L-Leu-4-nitroanilide + H2O
-
Ustilago maydis L-Leu + 4-nitroaniline
-
?
L-Lys-4-nitroanilide + H2O
-
Ustilago maydis L-Lys + 4-nitroaniline
-
?
additional information substrate specificity, the enzyme prefers Arg or Lys in N-terminal position, no activity with dipeptide-4-nitroanilides, overview Ustilago maydis ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 58000, SDS-PAGE Ustilago maydis

Synonyms

Synonyms Comment Organism
pumAPE
-
Ustilago maydis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Ustilago maydis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 80
-
Ustilago maydis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 88 1 h, loss of activity Ustilago maydis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Ustilago maydis

pH Range

pH Minimum pH Maximum Comment Organism
7 9 narrow pH-optimum Ustilago maydis

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9 purified enzyme, 4°C, 12 h, stable Ustilago maydis

pI Value

Organism Comment pI Value Maximum pI Value
Ustilago maydis isoelectric focusing
-
5.1