Literature summary for 3.4.11.5 extracted from

Li, N.; Wu, J.M.; Zhang, L.F.; Zhang, Y.Z.; Feng, H.
Characterization of a unique proline iminopeptidase from white-rot basidiomycetes Phanerochaete chrysosporium (2010), Biochimie, 92, 779-788.

Search for more literature for 3.4.11.5

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Phanerodontia chrysosporium

Protein Variants

Protein Variants	Comment	Organism
D264A	mutant shows no enzymatic activity	Phanerodontia chrysosporium
H292L	mutant shows no enzymatic activity	Phanerodontia chrysosporium
S107D	mutant shows no enzymatic activity	Phanerodontia chrysosporium

Inhibitors

Inhibitors	Comment	Organism	Structure
Al3+	slight inhibition	Phanerodontia chrysosporium
Ba2+	slight inhibition	Phanerodontia chrysosporium
benzamidine	moderate inhibition	Phanerodontia chrysosporium
Ca2+	slight inhibition	Phanerodontia chrysosporium
Co2+	strong inhibition	Phanerodontia chrysosporium
Cu2+	almost inactivates the enzyme	Phanerodontia chrysosporium
Fe2+	slight inhibition	Phanerodontia chrysosporium
Fe3+	slight inhibition	Phanerodontia chrysosporium
Hg2+	almost inactivates the enzyme	Phanerodontia chrysosporium
hydrochloride	moderate inhibition	Phanerodontia chrysosporium
Mg2+	slight inhibition	Phanerodontia chrysosporium
Mn2+	strong inhibition	Phanerodontia chrysosporium
Ni2+	strong inhibition	Phanerodontia chrysosporium
phenylmethanesulfonyl fluoride	moderate inhibition	Phanerodontia chrysosporium
Zn2+	almost inactivates the enzyme	Phanerodontia chrysosporium

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.15	-	proline-4-nitroanilide	Vmax: 347.86 micromol/min/mg	Phanerodontia chrysosporium

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
37620	-	calculated from cDNA	Phanerodontia chrysosporium
38000	-	SDS-PAGE	Phanerodontia chrysosporium

Organism

Organism	UniProt	Comment	Textmining
Phanerodontia chrysosporium	C6KI04	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-proline 4-nitroanilide + H2O	-	Phanerodontia chrysosporium	L-proline + 4-nitroaniline	-	?
additional information	the recombinant enzyme can cleave the peptides derived from enzyme-hydrolytic natural proteins to release free lysine	Phanerodontia chrysosporium	?	-	?

Synonyms

Synonyms	Comment	Organism
PchPiPA	-	Phanerodontia chrysosporium

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
45	-	optimal temperature and assay at	Phanerodontia chrysosporium

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
218.1	-	proline-4-nitroanilide	-	Phanerodontia chrysosporium

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	optimal pH and assay at	Phanerodontia chrysosporium

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Release 2023.1 (January 2023)