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Literature summary for 3.4.11.5 extracted from

  • Hiwatashi, K.; Hori, K.; Takahashi, K.; Kagaya, A.; Inoue, S.; Sugiyama, T.; Takahashi, S.
    Purification and characterization of a novel prolyl aminopeptidase from Maitake (Grifola frondosa) (2004), Biosci. Biotechnol. Biochem., 68, 1395-1397.
    View publication on PubMed
Search for more literature for 3.4.11.5

Inhibitors

Inhibitors Comment Organism Structure
arphamenine A
-
 Grifola frondosa
iodoacetic acid
-
 Grifola frondosa
p-chloromercuribenzoic acid
-
 Grifola frondosa
phenylmethylsulfonyl fluoride
-
 Grifola frondosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
 Gly-p-nitroanilide
-
 Grifola frondosa
0.24
-
 His-p-nitroanilide
-
 Grifola frondosa
0.34
-
 Ala-p-nitroanilide
-
 Grifola frondosa
0.73
-
 Pro-p-nitroanilide
-
 Grifola frondosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 2 * 33000, SDS-PAGRE, 2 * 34090 MALDI-TOF/MS analysis Grifola frondosa
34090
-
 2 * 33000, SDS-PAGRE, 2 * 34090 MALDI-TOF/MS analysis Grifola frondosa
58000
-
 gel filtration Grifola frondosa

Organism

Organism UniProt Comment Textmining
Grifola frondosa
-
 Maitake
-
Grifola frondosa Maitake
-
 Maitake
-

Purification (Commentary)

Purification (Comment) Organism
DEAE Sepharose CL6B, Butyl-Toyopearl, Sephacryl S-100 and Mono-Q column chromatographies Grifola frondosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly-p-nitroanilide + H2O
-
 Grifola frondosa glycine + p-nitroaniline
-
 ?
Gly-p-nitroanilide + H2O
-
 Grifola frondosa Maitake glycine + p-nitroaniline
-
 ?
His-p-nitroanilide + H2O
-
 Grifola frondosa L-histidine + p-nitroaniline
-
 ?
His-p-nitroanilide + H2O
-
 Grifola frondosa Maitake L-histidine + p-nitroaniline
-
 ?
L-Ala-p-nitroanilide + H2O
-
 Grifola frondosa L-Ala + p-nitroaniline
-
 ?
L-Ala-p-nitroanilide + H2O
-
 Grifola frondosa Maitake L-Ala + p-nitroaniline
-
 ?
Pro-p-nitroanilide + H2O
-
 Grifola frondosa L-proline + p-nitroaniline
-
 ?
Pro-p-nitroanilide + H2O
-
 Grifola frondosa Maitake L-proline + p-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 33000, SDS-PAGRE, 2 * 34090 MALDI-TOF/MS analysis Grifola frondosa

Synonyms

Synonyms Comment Organism
PAP
-
 Grifola frondosa
Prolyl aminopeptidase
-
 Grifola frondosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Grifola frondosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 more than 60% activity remained Grifola frondosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2 8 Ala-p-nitroanilide
-
 Grifola frondosa
6.5
-
 His-p-nitroanilide
-
 Grifola frondosa
7.8
-
 Gly-p-nitroanilide
-
 Grifola frondosa
160
-
 Pro-p-nitroanilide
-
 Grifola frondosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Grifola frondosa

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 9.5
-
 Grifola frondosa