Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoic acid | 61% remaining activity at 1 mM | Debaryomyces hansenii | |
Hg2+ | - |
Debaryomyces hansenii | |
iodoacetate | 2.6% remaining activity at 1 mM | Debaryomyces hansenii | |
additional information | not affected by bestatin, puromycin, reducing agents, chelating agents and cations | Debaryomyces hansenii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
proline-7-amido-4-methylcoumarin | pH 7.5, 37°C | Debaryomyces hansenii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53500 | - |
7 * 53500, SDS-PAGE | Debaryomyces hansenii |
370000 | - |
gel filtration | Debaryomyces hansenii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Debaryomyces hansenii | enzyme possibly plays a role in meat fermentation | ? | - |
? | |
additional information | Debaryomyces hansenii CECT12487 | enzyme possibly plays a role in meat fermentation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Debaryomyces hansenii | - |
- |
- |
Debaryomyces hansenii CECT12487 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
248fold | Debaryomyces hansenii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
release of N-terminal proline from a peptide | the enzyme exclusively hydrolyzes N-terminal-proline-containing substrates | Debaryomyces hansenii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.06 | - |
purified enzyme | Debaryomyces hansenii |
Storage Stability | Organism |
---|---|
4°C, purified enzyme, stable for at least 3 months | Debaryomyces hansenii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificity, no activity with any other Xaa-7-amido-4-methylcoumarin substrate, no activity with substrates Leu-Pro, Leu-Leu, Val-Val, Gly-Pro, Lys-Lys, Glu-Glu, and Ala-Ala-Ala | Debaryomyces hansenii | ? | - |
? | |
additional information | enzyme possibly plays a role in meat fermentation | Debaryomyces hansenii | ? | - |
? | |
additional information | substrate specificity, no activity with any other Xaa-7-amido-4-methylcoumarin substrate, no activity with substrates Leu-Pro, Leu-Leu, Val-Val, Gly-Pro, Lys-Lys, Glu-Glu, and Ala-Ala-Ala | Debaryomyces hansenii CECT12487 | ? | - |
? | |
additional information | enzyme possibly plays a role in meat fermentation | Debaryomyces hansenii CECT12487 | ? | - |
? | |
Pro-Gly + H2O | - |
Debaryomyces hansenii | Pro + Gly | - |
? | |
Pro-Gly + H2O | - |
Debaryomyces hansenii CECT12487 | Pro + Gly | - |
? | |
Pro-Leu + H2O | - |
Debaryomyces hansenii | Pro + Leu | - |
? | |
Pro-Leu + H2O | - |
Debaryomyces hansenii CECT12487 | Pro + Leu | - |
? | |
Pro-Phe + H2O | - |
Debaryomyces hansenii | Pro + Phe | - |
? | |
Pro-Phe + H2O | - |
Debaryomyces hansenii CECT12487 | Pro + Phe | - |
? | |
Pro-Phe-Gly-Lys + H2O | - |
Debaryomyces hansenii | Pro + Phe-Gly-Lys | - |
? | |
proline-7-amido-4-methylcoumarin + H2O | - |
Debaryomyces hansenii | proline + 7-amino-4-methylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
heptamer | 7 * 53500, SDS-PAGE | Debaryomyces hansenii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Debaryomyces hansenii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Debaryomyces hansenii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9.5 | - |
Debaryomyces hansenii |