Cloned (Comment) | Organism |
---|---|
- |
Lactobacillus helveticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 1 mM, complete inhibition | Lactobacillus helveticus | |
2-mercaptoethanol | 0.1 mM, 42% inhibition | Lactobacillus helveticus | |
Cd2+ | 0.1 mM, complete inhibition | Lactobacillus helveticus | |
Co2+ | 0.1 mM, 72% inhibition | Lactobacillus helveticus | |
Cu2+ | 0.1 mM, complete inhibition | Lactobacillus helveticus | |
dithiothreitol | 0.1 mM, 72% inhibition | Lactobacillus helveticus | |
EDTA | 0.1 mM, complete inhibition | Lactobacillus helveticus | |
EGTA | 0.1 mM, complete inhibition | Lactobacillus helveticus | |
Mn2+ | 0.1 mM, 53% inhibition | Lactobacillus helveticus | |
phenylmethylsulfonyl fluoride | 0.1 mM, 89% inhibition | Lactobacillus helveticus | |
Zn2+ | 1.0 mM, 91% inhibition | Lactobacillus helveticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
Leu-Gly-Gly | - |
Lactobacillus helveticus | |
2.6 | - |
Met-Gly-Gly | - |
Lactobacillus helveticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activation to 120% of control be 0.1 mM, activation to 168% of control by 1.0 mM | Lactobacillus helveticus | |
Zn2+ | activation to 112% of control by 0.1 mM | Lactobacillus helveticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
3 * 47000, SDS-PAGE | Lactobacillus helveticus |
150000 | - |
gel filtration | Lactobacillus helveticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus helveticus | Q9L4G1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Lactobacillus helveticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
0.0567 per mg per min | Lactobacillus helveticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Ala-Ala + H2O | 26% of the activity with Met-Gly-Gly | Lactobacillus helveticus | Ala + Ala-Ala | - |
? | |
Gly-Leu-Tyr + H2O | 15% of the activity with Met-Gly-Gly | Lactobacillus helveticus | Gly + Leu-Tyr | - |
? | |
Leu-Gly-Gly + H2O | 34% of the activity with Met-Gly-Gly | Lactobacillus helveticus | Leu + Gly-Gly | - |
? | |
Leu-Leu-Ala + H2O | 33% of the activity with Met-Gly-Gly | Lactobacillus helveticus | Leu + Leu-Ala | - |
? | |
Leu-Leu-Leu + H2O | 15% of the activity with Met-Gly-Gly | Lactobacillus helveticus | Leu + Leu-Leu | - |
? | |
Lys-Gly-Gly + H2O | 13% of the activity with Met-Gly-Gly | Lactobacillus helveticus | Lys + Gly-Gly | - |
? | |
Met-Gly-Gly + H2O | - |
Lactobacillus helveticus | Met + Gly-Gly | - |
? | |
Tyr-Gly-Gly + H2O | 11% of the activity with Met-Gly-Gly | Lactobacillus helveticus | Tyr + Gly-Gly | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 47000, SDS-PAGE | Lactobacillus helveticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
15 min, 55% loss of activity | Lactobacillus helveticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Lactobacillus helveticus |