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Literature summary for 3.4.11.4 extracted from
- A highly specific aminotripeptidase of rat brain cytosol. Substrate specificity and effects of inhibitors (1982), Biochim. Biophys. Acta, 706, 229-238.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| antipain | - |
Rattus norvegicus |  |
| bestatin | - |
Rattus norvegicus | |
| Cd2+ | - |
Rattus norvegicus | |
| chymostatin | - |
Rattus norvegicus | |
| Cu2+ | - |
Rattus norvegicus | |
| D-3-thio-2-methylpropanyl proline | - |
Rattus norvegicus | |
| iodoacetate | - |
Rattus norvegicus | |
| L-Leu | - |
Rattus norvegicus | |
| L-Leu-Gly-Gly | substrate inhibition | Rattus norvegicus | |
| Met-enkephalin | - |
Rattus norvegicus | |
| Met-Leu-Tyr | - |
Rattus norvegicus | |
| N-ethylmaleimide | - |
Rattus norvegicus | |
| Pb2+ | - |
Rattus norvegicus | |
| Phenylmethanesulfonylfluoride | - |
Rattus norvegicus | |
| tosyl-phenylalanine-chloromethylketone | - |
Rattus norvegicus | |
| Tripeptides with Pro in N-terminal position | - |
Rattus norvegicus | |
| Zn2+ | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
Leu-Gly-Gly | - |
Rattus norvegicus | |
| 0.9 | - |
Phe-Gly-Gly | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Rattus norvegicus | 5737 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tripeptides + H2O | Rattus norvegicus | e.g. Met-Leu-Tyr: chemotactic factor, Gly-His-Lys: liver growth factor, slow hydrolysis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 73.5 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 100 mM potassium phosphate buffer, pH 7.6, 0.05 M dithiothreitol, 50% glycerol | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Phe-Gly-Gly + H2O | - |
Rattus norvegicus | L-Phe + Gly-Gly | - |
? | |
| Leu-Gly-Gly + H2O | Leu can be replaced by Ala, Phe, Gly-Pro | Rattus norvegicus | Leu + Gly-Gly | - |
? | |
| additional information | - |
Rattus norvegicus | ? | - |
? | |
| tripeptides + H2O | e.g. Met-Leu-Tyr: chemotactic factor, Gly-His-Lys: liver growth factor, slow hydrolysis | Rattus norvegicus | ? | - |
? | |
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