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Literature summary for 3.4.11.26 extracted from

  • Naamati, A.; Regev-Rudzki, N.; Galperin, S.; Lill, R.; Pines, O.
    Dual targeting of Nfs1 and discovery of its novel processing enzyme, Icp55 (2009), J. BIOL. CHEM., 284, 30200-30208.
    View publication on PubMedView publication on EuropePMC

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
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Saccharomyces cerevisiae 5739
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nucleus
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Saccharomyces cerevisiae 5634
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Tyr-Ser-Pro-Nfs1 + H2O Saccharomyces cerevisiae the enzyme is involved in the processing pathway of yeast Nfs1 during its translocation into the mitochondrial matrix. Nfs1 from Saccharomyces cerevisiae undergoes two steps of proteolytic processing: first the mitochondrial processing peptidase (MPP) cleaves the precursor between Phe33 and Tyr34. Then Icp55 cleaves between Pro36 and Pro37 removing three amino acids residues (Tyr-Ser-Pro) Nfs1 + Tyr-Ser-Pro
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?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P40051
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Tyr-Ser-Pro-Nfs1 + H2O the enzyme is involved in the processing pathway of yeast Nfs1 during its translocation into the mitochondrial matrix. Nfs1 from Saccharomyces cerevisiae undergoes two steps of proteolytic processing: first the mitochondrial processing peptidase (MPP) cleaves the precursor between Phe33 and Tyr34. Then Icp55 cleaves between Pro36 and Pro37 removing three amino acids residues (Tyr-Ser-Pro) Saccharomyces cerevisiae Nfs1 + Tyr-Ser-Pro
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?
Tyr-Ser-Pro-Nfs1 + H2O the enzyme is involved in the processing pathway of yeast Nfs1 during its translocation into the mitochondrial matrix. Nfs1 from Saccharomyces cerevisiae undergoes two steps of proteolytic processing: first the mitochondrial processing peptidase (MPP) cleaves the precursor between Phe33 and Tyr34. Then Icp55 cleaves between Pro36 and Pro37 removing three amino acids residues (Tyr-Ser-Pro). In the absence of Nfs1 processing by mitochondrial processing peptidase (MPP), the Nfs1 precursor is not cleaved by Icp55. The two prolines are of importance for the Icp55 cleavage reaction, because the replacement of a single proline changes the specificity to a new cleavage site without eliminating the cleavage reaction per se Saccharomyces cerevisiae Nfs1 + Tyr-Ser-Pro
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?

Synonyms

Synonyms Comment Organism
Icp55
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Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction Nfs1 purified from the knockout strain, DELTAip55, detected three additional amino acids (Tyr-Ser-Pro), at the N-terminus of Nfs1 Saccharomyces cerevisiae